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Modification of a catalase by glutaraldehyde

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Abstract

Polycondensation of a catalase (EC 1.11.1.6) with glutaraldehyde in order to stabilize the quaternary structure of an enzyme, maintain its activity, and protect it from thermal denaturation was studied. Synthesis showed a superequivalent utilization of the aldehyde groups relative to the catalase amine groups, as a result of the formation of glutaraldehyde oligomers linked to the enzyme.

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References

  1. Plate, N.A. and Vasil’ev, A.E., Fiziologicheski Aktivnye Polimery (Physiologically Active Polymers), Moscow: Khimiya, 1986.

    Google Scholar 

  2. Fridovich, I., in Free Radicals in Biology, Pryor, W.A., Ed., New York: Academic, 1976, Translated under the title Svobodnye radikaly v biologii, Moscow: Mir, 1979, vol. 1, pp. 272–314.

    Google Scholar 

  3. Dubinina, E.E., Usp. Sovr. Biol., 1989, vol. 108, pp. 3–18.

    CAS  Google Scholar 

  4. Volker, H. and Fahimi, H.D., J. Cell. Biol., 1974, vol. 60, no. 1, pp. 303–311.

    Article  Google Scholar 

  5. Maksimenko, A.V., Usp. Sovr. Biol., 1993, vol. 113, pp. 351–365.

    CAS  Google Scholar 

  6. Metelitza, D.I., Plyugacheva, E.I., Artamonov, V.I., and Baran, G.M., Kinet. Katal., 1990, vol. 31, no. 6, pp. 1407–1413.

    Google Scholar 

  7. Metelitza, D.I., Plyugacheva, E.I., Ermolenko, I.N., Lyubliner, I.P., Kaputskii, F.N., and Pratsenko, V.E., Prikl. Biokhim. Mikrobiol., 1992, vol. 28, no. 4, pp. 531–537.

    Google Scholar 

  8. Miyahara, T., Takeda, A., Hashimore, A., and Samejma, T., J. Biochem., 1978, vol. 84, no. 5, pp. 1267–1276.

    PubMed  CAS  Google Scholar 

  9. Beers, R.F.J. and Sizer, J.W., J. Biol. Chem., 1952, vol. 195, no. 1, pp. 133–140.

    PubMed  CAS  Google Scholar 

  10. Wayne, F., Beyer, J.R., Fridovich, I., Anal. Biochem., 1987, vol. 161, no. 2, pp. 559–565.

    Article  Google Scholar 

  11. Fields, R., Biochem. J., 1971, vol. 124, no. 3, pp. 581–590.

    PubMed  CAS  Google Scholar 

  12. Roe, H.R. and Mitchel, G., Anal. Chem., 1951, vol. 23, no. 12, pp. 1758–1760.

    Article  CAS  Google Scholar 

  13. Boratynski, J. and Zal, T., Anal. Biochem., 1990, vol. 1844, no. 2, pp. 259–262.

    Article  Google Scholar 

  14. Kuznetsova, N.P. and Samsonov, G.V., Vysokomolek. Soed., 1985, vol. 27A, no. 12, pp. 2611–2614.

    Google Scholar 

  15. Hopwood, D., in Fixation in Histochemistry, Stoward, P.J., Ed., London: Chapman Hall, 1973, pp. 47–84.

    Google Scholar 

  16. Richard, F.M., Knowles, J.R., J. Mol. Biol., 1968, vol. 37, no. 1, pp. 231–233.

    Article  Google Scholar 

  17. Monsan, P., Puzo, G., and Mozarquil, H., Biochimie, 1975, vol. 57, nos. 11–12, pp. 1281–1292.

    PubMed  CAS  Google Scholar 

  18. Kuznetsova, N.P., Gudkin, L.R., Kol’tsova, S.V., and Mishaeva, R.N., Vysokomol. Soed., 1996, vol. 38A, no. 10, pp. 1668–1673.

    Google Scholar 

  19. Schroeder, W.A. and Shelton, Y.B., Arch. Biochem. Biophys., 1982, vol. 214, no. 2, pp. 397–421.

    Article  PubMed  CAS  Google Scholar 

  20. Kuznetsova, N.P., Mishaeva, R.N., and Gudkin, L.R., Zh. Prikl. Khim., 1999, vol. 72, no. 7, pp. 1171–1177.

    CAS  Google Scholar 

  21. Gudkin, L.R. and Kuznetsova, N.P., Zh. Prikl. Khim., 1997, vol. 70, no. 5, pp. 820–823.

    CAS  Google Scholar 

  22. Margel, S., J. Polym. Sci., 1984, vol. 22, no. 1, pp. 3521–3533.

    CAS  Google Scholar 

  23. Nakagawa, T., Izawa, K., and Jagi, S., Chem. Pharm. Bull., 1989, vol. 37, no. 9, pp. 2463–2466.

    PubMed  Google Scholar 

  24. Kuznetsova, N.P., Mishaeva, R.N., Pisarev, O.A., and Gudkin, L.R., Zh. Prikl. Khim., 2000, vol. 73, no. 5, pp. 796–801.

    CAS  Google Scholar 

  25. D’Agnillo, F. and Chang, T.M.S., Artific. Cells, Blood Subst. Immob., 1993, vol. 21, no. 5, pp. 609–621.

    CAS  Google Scholar 

  26. Mao, G.D., Thomas, P.D., Lopaschuk, G.D., and Posnansky, M.I., J. Biol. Chem., 1993, vol. 268, no. 1, pp. 416–420.

    PubMed  CAS  Google Scholar 

  27. Mishaeva, R.N. and Kuznetsova, N.P., Khim.-Farm. Zh., 1996, vol. 30, no. 4, pp. 6–10.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of High-Molecular-Weight Compounds, Russian Academy of Sciences, St. Petersburg, 199004, Russia

    R. N. Mishaeva, L. R. Gudkin & N. P. Kuznetsova

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  1. R. N. Mishaeva
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  2. L. R. Gudkin
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  3. N. P. Kuznetsova
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Additional information

Original Russian Text © R.N. Mishaeva, L.R. Gudkin, N.P. Kuznetsova, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 4, pp. 404–408.

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Mishaeva, R.N., Gudkin, L.R. & Kuznetsova, N.P. Modification of a catalase by glutaraldehyde. Appl Biochem Microbiol 42, 354–359 (2006). https://doi.org/10.1134/S0003683806040028

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  • DOI: https://doi.org/10.1134/S0003683806040028

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