Applied Biochemistry and Microbiology

, Volume 42, Issue 2, pp 200–203 | Cite as

Effect of the molecular weight of chitosan on its antiviral activity in plants

  • S. N. Kulikov
  • S. N. Chirkov
  • A. V. Il’ina
  • S. A. Lopatin
  • V. P. Varlamov


The effect of the molecular weight of chitosan on its ability to suppress systemic infection of bean mild mosaic virus in bean (Phaseolus vulgaris L.) plants was studied. The enzymatic hydrolysate of low-molecular-weight chitosan was successively fractionated by ultrafiltration through membranes with decreasing pore size. In total, four chitosan fractions with a weight-average molecular weight varying from 1.2 to 40.4 kDa were obtained. It was shown that the treatments of bean plants with these fractions (chitosan concentration, 10 or 100 μg/ml) inhibited virus accumulation and systemic propagation. The degree of chitosan-induced antiviral resistance increased as the molecular weight of chitosan decreased. The monomers comprising the chitosan molecule—glucosamine and N-acetylglucosamine—exhibited no antiviral activity.


Molecular Weight Chitosan Pore Size Enzymatic Hydrolysate Mosaic Virus 
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Copyright information

© MAIK “Nauka/Interperiodica” 2006

Authors and Affiliations

  • S. N. Kulikov
    • 1
  • S. N. Chirkov
    • 2
  • A. V. Il’ina
    • 1
  • S. A. Lopatin
    • 1
  • V. P. Varlamov
    • 1
  1. 1.Bioengineering CenterRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of BiologyMoscow State UniversityMoscowRussia

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