Conjugate symbiotic populations part II: Analysis of nfr5 receptor gene polymorphisms using molecular docking
In this study, an analysis of nucleotide and amino-acid polymorphisms in the receptor part of the nfr5 gene was conducted in 15 plants of the conjugate collection (Melilotus albus was the plant component; Sinorhizobium meliloti was the bacterial component). The domain structure of the studied region was determined and it was demonstrated that the maxima of the nucleotide polymorphisms fall on the LysM domains of this gene. A clear association between nucleotide and amino-acid polymorphisms of the plant receptor region of the nfr5 gene and the genetic diversity of the rhizobial component of the conjugate system (symbiotic nodD locus) was detected. Based on the data that were obtained, computer modeling of bacterial Nod factor binding with two variants of the plant receptor was conducted.
Keywordsconjugate collection nfr5 Nod factor Melilotus albus molecular modeling docking
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