Advertisement

Advances in Gerontology

, Volume 9, Issue 1, pp 75–80 | Cite as

The Influence of Peptides on the Morphofunctional State of Kidneys in Old Rats

  • I. I. ZamorskiiEmail author
  • T. S. Shchudrova
  • V. G. Zeleniuk
  • N. S. Linkova
  • T. E. Nichik
  • V. Kh. KhavinsonEmail author
Article

Abstract

More than a quarter of the elderly and senile age population suffers from kidney pathology. For this reason, a prophylaxis of kidney diseases with safe and effective nephroprotectors is a priority of gerontology. This work studies the influence of the polypeptide kidney complex (PKC) and peptides AED, EDL, and AEDG, on the functional state of kidneys in old rats. The administration of the PKC and peptides AED and EDL increased diuresis by 1.2–1.4 times. The PKC and peptide AED reduced the urine protein level and protein excretion by 1.5–2.8 times. The PKC and peptides AED and EDL increased distal sodium transport by 1.2–1.3 times. The peptides AED and EDL increased sodium excretion by 1.3 and 1.6 times respectively. The renal effects of the peptide AEDG resulted in a 21% reduction of glomerular filtration rate, a 3.1-fold decrease in the urine protein level, and a 2.5-fold decrease in protein excretion. The peptide AEDG reduced absolute sodium reabsorption by 1.3 times and increased distal sodium transport by 1.4 times. The realization of glomerular–tubular and tubular–tubular balances is verified by the correlation between the glomerular filtration rate (GFR) and absolute, proximal, and distal sodium reabsorption. In kidney tissue, stimulation of the antioxidant enzyme activity on the background of inhibition of the intensity peroxidation processes was observed, which, together with morphological data, reflects the absence of nephrotoxic effects. PKC and peptides AED, EDL, and AEDG may be considered nephroprotective agents in kidney aging.

Keywords:

peptides renal effects nephroprotection aging 

Notes

REFERENCES

  1. 1.
    Arutyunyan, A.V., Dubinina, E.E., and Zybina, N.N., Metody otsenki svobodnoradikal’nogo okisleniya i antioksidantnoi sistemy organizma: Metodicheskie rekomendatsii (Evaluation of Free Radical Oxidation and Antioxidant System of Organism: Methodological Recommendations), St. Petersburg, 2000.Google Scholar
  2. 2.
    Zamorskii, I.I. and Shchudrova, T.S., Main mechanisms of rhabdomyolysis-caused kidney injury and their correction by organospecific peptides, Biophysics (Moscow), 2014, vol. 59, no. 5, pp. 834–836.Google Scholar
  3. 3.
    Zamorskii, I.I., Shchudrova, T.S., Lin’kova, N.S., Nichik, T.E., and Khavinson, V.K., Peptides restore functional state of the kidneys during cisplatin-induced acute renal failure, Bull. Exp. Biol. Med., 2015, vol. 159, no. 6, pp. 736–739.CrossRefGoogle Scholar
  4. 4.
    Zamorskii, I.I., Shchudrova, T.S., Lin’kova, N.S., Nichik, T.E., and Khavinson, V.K., Nephroprotective effect of EDL peptide at acute injury of kidneys of different genesis, Bull. Exp. Biol. Med., 2017, vol. 163, no. 3, pp. 389–393.CrossRefGoogle Scholar
  5. 5.
    Metody klinicheskikh laboratornykh issledovanii (Methods of Clinical Laboratory Studies), Kamyshnikov, V.S., Ed., Moscow: MEDpressinform, 2016, 8th ed.Google Scholar
  6. 6.
    Ryzhak, A.P., Chalisova, N.I., Lin’kova, N.S., et al., Effect of polypeptides on cell proliferation and apoptosis during aging, Bull. Exp. Biol. Med., 2017, vol. 162, no. 4, pp. 534–538.CrossRefGoogle Scholar
  7. 7.
    Khavinson, V.Kh., Lin’kova, N.S., Polyakova, V.O., Durnova, A.O., Nichik, T.E., and Kvetnoi, I.M., Peptides regulate expression of signaling molecules in kidney cell cultures during in vitro aging, Bull. Exp. Biol. Med., 2014, vol. 157, no. 2, pp. 261–264.CrossRefGoogle Scholar
  8. 8.
    Chalisova, N.I., Lin’kova, N.S., Nichik, T.E., et al., Peptide regulation of cells renewal processes in kidney tissue cultures from young and old animals, Bull. Exp. Biol. Med., 2015, vol. 159, no. 1, pp. 124–127.CrossRefGoogle Scholar
  9. 9.
    Shtrygol’, S.Yu., Tovchiga, O.V., Koiro, O.O., and Shchekina, E.G., New perspectives of nephroprotection, Bukovins’kii Med. Visn., 2012, vol. 16, no. 3 (63), pp. 35–37.Google Scholar
  10. 10.
    Ahmad, Q.Z., Jahan, N., Ahmad, G., and Tajuddin, J., Appraisal of nephroprotection and the scope of natural products in combating renal disorders, Nephrol. Ther., 2014, vol. 4, no. 170.  https://doi.org/10.4172/2161-0959.1000170
  11. 11.
    Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes, Off. J. Eur. Union, 2010, vol. 53, no. L276/33, pp. 33–80. doi 10.3000/17252555.L_2010.276.engGoogle Scholar
  12. 12.
    Gansevoort, R.T., Correa-Rotter, R., Hemmelgarn, B.R., et al., Chronic kidney disease and cardiovascular risk: epidemiology, mechanisms, and prevention, Lancet, 2013, vol. 382, pp. 339–352.CrossRefGoogle Scholar
  13. 13.
    Hill, N.R., Fatoba, S.T., Oke, J.L., et al., Global prevalence of chronic kidney disease—a systematic review and meta-analysis, PLoS One, 2016, vol. 11, no. 7, p. e0158765.  https://doi.org/10.1371/journal.pone.0158765 CrossRefGoogle Scholar
  14. 14.
    Jha, V., Garcia-Garcia, G., Iseki, K., et al., Chronic kidney disease: global dimension and perspectives, Lancet, 2013, vol. 382, pp. 260–272.CrossRefGoogle Scholar
  15. 15.
    Khavinson, V.Kh., EU Patent 1 179 008, 2006.Google Scholar
  16. 16.
    Khavinson, V.Kh., Grigoriev, E.I., Malinin, V.V. and Ryzhak, G.A., EA Patent 010 156, 2008.Google Scholar
  17. 17.
    Khavinson, V.Kh., Grigoriev, E.I., Malinin, V.V. and Ryzhak, G.A., EA Patent 010 574, 2008.Google Scholar
  18. 18.
    Khavinson, V.Kh., Malinin, V.V. and Ryzhak, G.A., EA Patent 010723, 2008.Google Scholar
  19. 19.
    Matsushita, K., van der Velde, M., Astor, B.C., et al., Association of estimated glomerular filtration rate and albuminuria with all-cause and cardiovascular mortality: a collaborative meta-analysis of general population cohorts, Lancet, 2010, vol. 375, pp. 2073–2081.  https://doi.org/10.1016/S0140-6736(10)60674-5 CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • I. I. Zamorskii
    • 1
    Email author
  • T. S. Shchudrova
    • 1
  • V. G. Zeleniuk
    • 1
  • N. S. Linkova
    • 2
    • 3
  • T. E. Nichik
    • 2
  • V. Kh. Khavinson
    • 2
    • 4
    Email author
  1. 1.Bukovinian State Medical UniversityChernovtsyUkraine
  2. 2.St. Petersburg Institute of Bioregulation and GerontologySt. PetersburgRussia
  3. 3.Peter the Great St. Petersburg Polytechnic UniversitySt. PetersburgRussia
  4. 4.Pavlov Institute of Physiology, Russian Academy of SciencesSt. PetersburgRussia

Personalised recommendations