The Influence of Metformin on Age-Related Changes in the Number and Proliferation of Dermal Fibroblasts in Mice
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The goal of the work was to examine the effects of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice. The dermis was examined in 5-month-old mice that received drinking water with metformin at a concentration of 500 mg/L from the age of 2 months (within 90 days). Five-month-old mice that received drinking water without metformin were used as a control. Material from 2-month-old mice was also used in the work. We have counted the total number of dermal fibroblasts and a portion of fibroblasts with positive staining for the Ki-67 antigen. The results showed that there was a decrease in the total number of mouse dermal fibroblasts by 42.3% from two up to 5 months of life and in the proportion of fibroblasts with positive staining for Ki-67 by 12%. The total number of dermal fibroblasts in 5-month-old mice was 13.4% lower under the effect of metformin. The proportion of fibroblasts with positive staining for Ki-67 was reduced by 27.3% as compared to animals that did not receive metformin. Thus, the age-related decrease in dermal fibroblasts of mice is due to the decline in their proliferation activity. Metformin has an inhibitory effect on the proliferation of dermal fibroblasts in mice.
Keywords:dermis fibroblasts metformin Ki-67 proliferation
This study was supported by the Russian Foundation for Basic Research, projects nos. 16-44-210018 and 18-04-00024.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study was approved by the Ethics Committee at the Faculty of Medicine (Ulyanov Chuvash State University).
- 1.Anisimov, V.N., Antidiabetic biguanide metformin as geroprotector and anticarcinogen, Ozhirenie Metab., 2011, no. 3, pp. 18–24.Google Scholar
- 2.Anisimov, V.N., Popovich, I.G., Egormin, P.A., et al., Use of antidiabetic biguanides for the prevention and treatment of cancer: the results of preclinical trials, Vopr. Onkol., 2016, vol. 62, no. 2, pp. 234–244.Google Scholar
- 5.Chetina, E.V., Nutrient signaling pathways and rheumatic diseases, Nauchno-Prakt. Revmatol., 2013, vol. 51, no. 3, pp. 313–323.Google Scholar