Advances in Gerontology

, Volume 3, Issue 3, pp 189–194 | Cite as

Age-related changes in number of CD45-positive cells in human dermis

  • V. V. Petrov
  • O. V. Vasil’eva
  • N. K. Kornilova
  • A. G. Gunin


Age-related changes in the number of leucocytes and other cells of bone marrow origin were investigated in human dermis. The total number of CD45-positive cells increased with age, while the amount of fibroblasts in the dermis gradually decreased. Additionally, an age-related decrease in the number of PCNA-positive fibroblast-like cells, which indicates their proliferative activity, was clearly shown. The correlation analysis revealed that the age-associated increase in the amount of CD45-positive cells is statistically related to a decrease in the total number of PCNA-positive fibroblast-like cells in the dermis. Therefore, the accumulation of CD45-positive cells in the human dermis with age may well be regarded as a mechanism that initiates an inflammatory response and is responsible for the emergence of signs of aging. It is also possible that an increased number of bone marrow-derived cells in the dermis, which are accumulated with age, has an effect on the age-related decrease in the amount of fibroblasts in the dermis.


skin aging CD45 fibroblasts proliferation PCNA 


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  1. 1.
    Bonafe, M., Storei, C., and Franceschi, C., Inflammaging of the stem cell niche: breast cancer as a paradigmatic example: breakdown of the multi-shell cytokine network fuels cancer in aged people, Bioessays, 2012, vol. 34, pp. 40–49.CrossRefPubMedGoogle Scholar
  2. 2.
    Dall’olio, F., Vanhooren, V., Chen, C.C., et al., N-glycomic biomarkers of biological aging and longevity: a link with inflamm-aging, Aging Res. Rev., 2012. doi: 10.1016/j.arr.2012.02.002Google Scholar
  3. 3.
    Ekiz, O., Yüce, G., Ulasli, S.S., et al., Factors influencing skin ageing in a Mediterranean population from Turkey, Clin. Exp. Dermatol., 2012, vol. 37, pp. 492–496.CrossRefPubMedGoogle Scholar
  4. 4.
    Gunin, A.G., Kapitova, I.N., and Suslonova, N.V., Effects of histone deacetylase inhibitors on estradiol-induced proliferation and hyperplasia formation in the mouse uterus, J. Endocrinol., 2005, vol. 185, pp. 539–549.CrossRefPubMedGoogle Scholar
  5. 5.
    Gunin, A.G., Bitter, A.D., Demakov, A.B., et al., Effects of peroxisome proliferator activated receptors α and γ agonists on estradiol-induced proliferation and hyperplasia formation in the mouse uterus, J. Endocrinol., 2004, vol. 182, pp. 229–239.CrossRefPubMedGoogle Scholar
  6. 6.
    Harvima, I.T. and Nilsson, G., Mast cells as regulators of skin inflammation and immunity, Acta Derm. Venereol., 2011, vol. 91, pp. 644–650.PubMedGoogle Scholar
  7. 7.
    Hwang, K.A., Yi, B.R., and Choi, K.C., Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations, ILAR J., 2011, vol. 27, pp. 1–8.Google Scholar
  8. 8.
    Kondo, T. and Ishida, Y., Molecular pathology of wound healing, Forensic. Sci. Int., 2010, vol. 203, pp. 93–98.CrossRefPubMedGoogle Scholar
  9. 9.
    Krtolica, A. and Campisi, J., Integrating epithelial cancer, aging stroma and cellular senescence, Adv. Gerontol., 2003, vol. 11, pp. 109–116.PubMedGoogle Scholar
  10. 10.
    Labat-Robert, J., Information exchanges between cells and extracellular matrix. Influence of aging, Biol. Aujourdhui, 2012, vol. 206, pp. 103–109.CrossRefPubMedGoogle Scholar
  11. 11.
    Lee, Y.K., Cha, H.J., Hong, M., et al., Role of NF-κB-p53 crosstalk in ultraviolet A-induced cell death and G1 arrest in human dermal fibroblasts, Arch. Dermatol. Res., 2012, vol. 304, pp. 73–79.CrossRefPubMedGoogle Scholar
  12. 12.
    Levakov, A., Vuckovic, N., Dolai, M., et al., Agerelated skin changes, Med. Pregl., 2012, vol. 65, pp. 191–195.CrossRefPubMedGoogle Scholar
  13. 13.
    Oh, J.H., Kim, Y.K., Jung, J.Y., et al., Changes in glycos-aminoglycans and related proteoglycans in intrinsically aged human skin in vivo, Exp. Dermatol., 2011, vol. 20, pp. 454–456.CrossRefPubMedGoogle Scholar
  14. 14.
    Quan, T., Qin, Z, Robichaud, P., et al., CCN1 contributes to skin connective tissue aging by inducing age-associated secretory phenotype in human skin dermal fibroblasts, J. Cell Commun. Signaling, 2011, vol. 5, pp. 201–207.CrossRefGoogle Scholar
  15. 15.
    Rijken, F. and Bruijnzeel, P.L., The pathogenesis of photo-aging: the role of neutrophils and neutrophilderived enzymes, J. Invest. Dermatol. Symp. Proc., 2009, vol. 14, pp. 67–72.CrossRefGoogle Scholar
  16. 16.
    Robert, L., Labat-Robert, J., and Robert, A.M., Physiology of skin aging, Clin. Plast. Surg., 2012, vol. 9, pp. 1–8.CrossRefGoogle Scholar
  17. 17.
    Saunders, A.E. and Johnson, P., Modulation of immune cell signaling by the leukocyte common tyrosine phosphatase, CD45, Cell Signaling, 2010, vol. 22, pp. 339–348.CrossRefGoogle Scholar
  18. 18.
    Sveikata, K., Balciuniene, I., and Tutkuviene, J., Factors influencing face aging. Literature review, Stomatologija, 2011, vol. 13, pp. 113–116.PubMedGoogle Scholar
  19. 19.
    Vukmanovic-Stejic, M., Rustin, M.H., Nikolich-Zugich, J., and Akbar, A.N., Immune responses in the skin in old age, Curr. Opin. Immunol., 2011, vol. 23, pp. 525–531.CrossRefPubMedGoogle Scholar
  20. 20.
    Wang, B., Photoaging: a review of current concepts of pathogenesis, J. Cutaneous Med. Surg., 2011, vol. 15, pp. S374–S377.Google Scholar
  21. 21.
    Wang, Y., Sun, Y., Yang, X.Y., et al., Mobilized bone marrow-derived cells accelerate wound healing, Int. Wound. J., 2012. doi: 10.1111/j.1742-481X.2012.01007.xGoogle Scholar
  22. 22.
    Wolf, J., Weinberger, B., Arnold, C.R., et al., The effect of chronological age on the inflammatory response of human fibroblasts, Exp. Gerontol., 2012, vol. 47, pp. 749–753.CrossRefPubMedGoogle Scholar
  23. 23.
    Zapata, A.G., Alfaro, D., and Garcia-Ceca, J., Biology of stem cells: the role of microenvironments, Adv. Exp. Med. Biol., 2012, vol. 741, pp. 135–151.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • V. V. Petrov
    • 1
  • O. V. Vasil’eva
    • 1
  • N. K. Kornilova
    • 1
  • A. G. Gunin
    • 1
  1. 1.Chuvash State UniversityCheboksaryRussia

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