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Contemporary Problems of Ecology

, Volume 11, Issue 6, pp 594–603 | Cite as

Effect of Secondary Metabolites Juniperus sabina L. (Cupressaceae) on the Survival and Nutrition of Galleria mellonella L. (Pyralidae)

  • D. S. ElisovetcaiaEmail author
  • J. Brindza
Article
  • 11 Downloads

Abstract

The effect of alcohol extract and essential oil from Juniperus sabina L. (Cupressaceae) on the survival and nutrition of the larvae Galleria mellonella L. (Pyralidae) has been studied. It is found that the essential oil has insecticidal, antifeedant, deterrent, and repellent properties. The loss of larvae with topical application of 0.2 μL of essential oil to the dorsal part (contact action) reaches 64.0%. Feeding larvae with food treated with essential oil J. sabina (200 μL of essential oil per 1.5 g of feed) is reduced 2.1 times compared to the control. It is shown that larvae G. mellonella avoid feeding on oil-treated feed for 14 days and actively move in the opposite direction from the treated feed when they are placed in Petri dishes. It has been established that vapors of essential oil suppress the development of larvae G. mellonella even when fed with untreated food—there is a lag in body weight and a delay in molting in the next age. The essential oil J. sabina also exhibits moderate toxicity (20.0%) when the larvae eat the treated food, i.e., possess intestinal action. The alcohol extract of J. sabina at a concentration of 2.5% of active substances (on dry residue) has a negligible effect on the loss of larvae in the case of treated feed (10.0% contact action). With topical application to the dorsal part, the extract causes 20.0% insect death. An alcohol extract of J. sabina does not elicit any antifeedant or repellent properties against larvae; however, a low level of deterrental activity, expressed in suppressing the formation and development of pupae of Galleria mellonella, is noted. Essential oil reduces the percentage of pupated larvae and emitted adults 3 times and the mass of larvae 40.7–48.0% when compared with the control.

Keywords

extract essential oil repellent antifeedant insecticide deterrent 

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References

  1. Artemkina, N.A. and Roshchin, V.I., Polar extractive substances from needles and sprouts of Norway spruce Picea abies (L.) Karst, Rastit. Resur., 2004, vol. 40, no. 3, pp. 77–86.Google Scholar
  2. Elisovetskaya, D., Nastas, T., Bucatel, V., and Galupa, D., Natural crop protection based on plant resources of the Juniperus sabina L. in the Republic of Moldova, Int. Sc. Symp. “Conservation of Plant diversity,” Chisinau: Bot. Gard., Univ. Acad. Sci. Mold., 2014, pp. 88–89. ISBN 978-9975-62-370-4.Google Scholar
  3. Elisovetskaya, D., Nastas, T., Bucatel, V., and Galupa, D., Natural crop protection based on plant resources of the Juniperus sabina L. in the Republic of Moldova, J. Bot., 2015, vol. 7, no. 10, pp. 65–70.Google Scholar
  4. Evtushenko, E.V., Saprykin, V.A., Galitsyn, M.Yu., and Chekurov, V.M., The effect of biologically active substances from coniferous plants on the L-phenylalanine ammonia lyase and peroxidase activities in wheat leaves, Appl. Biochem. Microbiol., 2008, vol. 44, no. 1, pp. 110–115.CrossRefGoogle Scholar
  5. Gao, R., Gao, C., Tian, X., Yu, X., Di, X., Xiao, H., and Zhang, X., Insecticidal activity of deoxypodophyllotoxin, isolated from Juniperus sabina L., and related lignans against larvae of Pieris rapae L., Pest Manage. Sci., 2004, vol. 60, pp. 1131–1136.CrossRefGoogle Scholar
  6. Graskova, I.A., Kuznetsova, E.V., Zhivetiev, M.A., Chekurov, V.M., and Voinikov, V.K., Effect of coniferous extract on potato plants, Zh. Stress-Fiziol. Biokhim., 2009, vol. 5, nos. 1–2, pp. 38–44.Google Scholar
  7. Hesham, H.A. Rassem Abdurahman, H., Nour, R., and Yunus, M., Techniques for extraction of essential oils from plants: a review, Aust. J. Basic Appl. Sci., 2016, vol. 10, no. 16, pp. 117–127. ISSN 1991–8178, EISSN 2309–8414.Google Scholar
  8. Jafari, R., Goldasteh, S., and Afrogheh, S., Control of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) by the male sterile technique (MST), Arch. Biol. Sci., Belgr., 2010, vol. 62, no. 2, pp. 309–313. doi 309 doi 10.2298/ABS1002309JCrossRefGoogle Scholar
  9. Khanturgaev, A.G., Badmatsyrenov, B.V., Shiretorova, V.G., and Khanturgaeva, G.I., Production of cedar oil from Siberian pine seeds by ethyl alcohol extraction, Izv. Vyssh. Uchebn. Zaved., Pishch. Tekhnol., 2003, no. 1, pp. 34–37.Google Scholar
  10. Khurshkainen, T.V. and Kuchin, A.V., Forest chemistry for innovations in agriculture, Izv. Komi Nauch. Tsentra, Ural. Otd., Ross. Akad. Nauk, 2011, no. 1, pp. 17–23.Google Scholar
  11. Khurshkainen, T.V., Skripova, N.N., and Kuchin, A.V., Highly effective technology of complex processing of plant raw materials and production of preparations for agriculture, Teor. Prikl. Ekol., 2007, no. 1, pp. 74–77.Google Scholar
  12. Konovalova, T.V., Laboratory growing of the greater wax moth Galleria mellonella L., Ross. Vet. Zh., S-kh. Zhivotn., 2009, no. 4, pp. 46–48.Google Scholar
  13. Kwadha, C.A., Ong’amo, G.O., Ndegwa, P.N., Raina, S.K., and Fombong, A.T., The biology and control of the greater wax moth, Galleria mellonella, Insects, 2017, vol. 8, no. 2, pp. 61–100. doi 10.3390/insects8020061CrossRefGoogle Scholar
  14. Markova, O.M., Karpenko, V.A., Saushkina, A.S., and Likhota, T.T., Physical-chemical analysis of medical drugs from the plant material, Vestn. Voronezh. Gos. Univ., Ser. Khim., Biol., Farm., 2003, no. 1, pp. 99–100.Google Scholar
  15. Mohamed, A.A., Ansari, M.J., Al-Ghamdi, A., Mohamed, M.O., and Kaur, M.T., Effect of larval nutrition on the development and mortality of Galleria mellonella (Lepidoptera: Pyralidae), Rev. Colomb. Entomol., 2014, vol. 40, no. 1, pp. 49–54.Google Scholar
  16. Myrzagalieva, A.B. and Medeubaeva, B.Z., The ether-oil composition of the members of family Cupressaceae Bartl. in flora of Eastern Kazakhstan, Fundam. Issled., 2014, no. 5–5, pp. 1021–1024.Google Scholar
  17. Nikolic, B., et al., Screening of the antibacterial effect of Juniperus sibirica and Juniperus sabina essential oils in a microtitre plate-based MIC assay, Bot. Serb., 2016, vol. 40, no. 1, pp. 43–48.Google Scholar
  18. Nosov, A.M., Lekarstvennye rasteniya (Medical Plants), Moscow, 2001.Google Scholar
  19. Osokina, A.S., Possible use of the greater wax moth (Galleria mellonella L.) grown in laboratory conditions, Cand. Sci. (Biol.) Dissertation, Izhevsk, 2016.Google Scholar
  20. Shirokikh, I.G., Ogorodnikova, S.Yu., Shirokikh, A.A., et al., Biological activity of terpenoids obtained by innovative processing technology of wood green of Siberian spruce (Picea obovata L.), Siberian fir (Abies sibirica L.), and silver birch (Betula pendula L.), Agrokhimiya, 2008, no. 10, pp. 1–8.Google Scholar
  21. Vasil’ev, S.N., Roshchin, V.I., and Feleke, S., Extracts from wood greens of Picea abies (L.) Karst, Rastit. Resur., 1996, nos. 1–2, pp. 151–175.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.Institute of GeneticsPhysiology and Plant Protection ASM, ChisinauChisinauRepublic of Moldova
  2. 2.Slovak University of Agriculture in NitraNitraSlovakia

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