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Comparative Analysis of Gene Expression in Vascular Cells of Patients with Advanced Atherosclerosis

  • M. S. NazarenkoEmail author
  • A. V. Markov
  • A. A. Sleptcov
  • I. A. Koroleva
  • D. V. Sharysh
  • A. A. Zarubin
  • N. R. Valiahmetov
  • I. A. Goncharova
  • E. F. Muslimova
  • M. S. Kuznetsov
  • B. N. Kozlov
  • S. A. Afanasiev
  • V. P. Puzyrev
Article
  • 23 Downloads

Abstract

A comparative analysis of gene expression profiles of carotid atherosclerotic plaques and intact internal thoracic arteries of patients with advanced atherosclerosis was performed by using the Human-12 BeadChip Microarray (Illumina, USA). The most down-regulated genes in the carotid atherosclerotic plaques were APOD, FABP4, CIDEC, and FOSB, in contrast, up-regulated gene was SPP1 (|FC| > 64; pFDR < 0.05). The majority of differentially expressed genes were down-regulated in advanced atherosclerotic plaques. For example, genes involved in immune and inflammatory responses (arachidonic acid metabolism, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, Jak-STAT signaling pathway, TNF signaling pathway) were down-regulated in advanced atherosclerotic plaques as compared with the healthy arteries. The most significant biological process of genes down-regulated in carotid atherosclerotic plaques (compared to intact internal thoracic arteries) was “cellular response to metal ions” (metallothioneins), and for upregulated genes was the organization of the extracellular matrix.

Key words:

gene expression carotid atherosclerosis vascular tissues microarrays 

Notes

REFERENCES

  1. 1.
    Nai, W., Threapleton, D., Lu, J., Zhang, K., Wu, H., Fu, Y., Wang, Y., Ou, Z., Shan, L., Ding, Y., Yu, Y., and Dai, M., Sci. Rep., 2016, vol. 6, 18 764.  https://doi.org/10.1038/srep18764 CrossRefGoogle Scholar
  2. 2.
    Sulkava, M., Raitoharju, E., Levula, M., Seppälä, I., Lyytikäinen, L.P., Mennander, A., Järvinen, O., Zeitlin, R., Salenius, J.P., Illig, T., Klopp, N., Mononen, N., Laaksonen, R., Kähönen, M., Oksala, N., and Lehtimäki, T., Sci. Rep., 2017, vol. 7, 41483.  https://doi.org/10.1038/srep41483 CrossRefGoogle Scholar
  3. 3.
    Steenman, M., Espitia, O., Maurel, B., Guyomarch, B., Heymann, M.F., Pistorius, M.A., Ory, B., Heymann, D., Houlgatte, R., Gouëffic, Y., and Quillard, T., Sci. Rep., 2018, vol. 8, 3940.  https://doi.org/10.1038/s41598-018-22292-y CrossRefGoogle Scholar
  4. 4.
    Stary, H.C., Chandler, A.B., Dinsmore, R.E., Fuster, V., Glagov, S., Insull, W., Jr., Rosenfeld, M.E., Schwartz, C.J., Wagner, W.D., and Wissler, R.W., Circulation, 1995, vol. 92, no. 5, pp. 1355–1374.CrossRefGoogle Scholar
  5. 5.
    Raitoharju, E., Seppälä, I., Lyytikäinen, L.P., Levula, M., Oksala, N., Klopp, N., Illig, T., Laaksonen, R., Kähönen, M., and Lehtimäki, T., Atherosclerosis, 2013, vol. 226, no. 1, pp. 149–152.  https://doi.org/10.1016/j.atherosclerosis.2012.10.078 CrossRefGoogle Scholar
  6. 6.
    Wang J., Duncan D., Shi Z., Zhang B., Nucleic Acids Res., 2013, vol. 41, W77–W83.  https://doi.org/10.1093/nar/gkt439
  7. 7.
    Patino, W.D., Kang, J.G., Matoba, S., Mian, O.Y., Gochuico, B.R., and Hwang, P.M., Circ. Res., 2006, vol. 98, no. 10, pp. 1282–1289.  https://doi.org/10.1161/01.RES.0000222284.48288.28 CrossRefGoogle Scholar
  8. 8.
    Thomas, A.C., Eijgelaar, W.J., Daemen, M.J., and Newby, A.C., PLoS One, 2015, vol. 10, no. 7, e0128163.  https://doi.org/10.1371/journal.pone.0128163 CrossRefGoogle Scholar
  9. 9.
    Zenz, R., Eferl, R., Scheinecker, C., Redlich, K., Smolen, J., Schonthaler, H.B., Kenner, L., Tschachler, E., and Wagner, E.F., Arthritis Res. Ther., 2008, vol. 10, no. 1, 201.  https://doi.org/10.1186/ar2338 CrossRefGoogle Scholar
  10. 10.
    Seo, D., Wang, T., Dressman, H., Herderick, E.E., Iversen, E.S., Dong, C., Vata, K., Milano, C.A., Rigat, F., Pittman, J., Nevins, J.R., West, M., and Goldschmidt-Clermont, P.J., Arterioscler. Thromb. Vasc. Biol., 2004, vol. 24, no. 10, pp. 1922–1927.  https://doi.org/10.1161/01.ATV.0000141358.65242.1f CrossRefGoogle Scholar
  11. 11.
    Antonopoulos, A.S., Margaritis, M., Shirodaria, C., and Antoniades, C., Thromb. Haemost., 2012, vol. 108, pp. 840–848.CrossRefGoogle Scholar
  12. 12.
    Dichtl, W., Dulak, J., Frick, M., Alber, H.F., Schwarzacher, S.P., Ares, M.P., Nilsson, J., Pachinger, O., and Weidinger, F., Arterioscler. Thromb. Vasc. Biol., 2003, vol. 23, no. 1, pp. 58–63.CrossRefGoogle Scholar
  13. 13.
    Blaschke, S., Viereck, V., Schwarz, G., Klinger, H.M., Guerluek, S., and Muller, G.A., Scand. J. Rheumatol., 2009, vol. 38, no. 4, pp. 235–239.CrossRefGoogle Scholar
  14. 14.
    Daskalopoulou, S.S., Daskalopoulos, M.E., Theocharis, S., Kavantzas, N., Perrea, D., Karandrea, D., Constantinides, A.G., Mikhailidis, D.P., Nicolaides, A.N., and Liapis, C.D., Curr. Med. Res. Opin., 2007, vol. 23, no. 3, pp. 659–670.  https://doi.org/10.1185/030079907X178829 CrossRefGoogle Scholar
  15. 15.
    Mocchegiani, E., Giacconi, R., Cipriano, C., Muzzioli, M., Gasparini, N., Moresi, R., Stecconi, R., Suzuki, H., Cavalieri, E., and Mariani, E., Exp. Gerontol., 2002, vol. 37, nos. 2–3, pp. 349–357.CrossRefGoogle Scholar
  16. 16.
    Brazão-Silva, M.T., Rodrigues, M.F., Eisenberg, A.L., Dias, F.L., de Castro, L.M., Nunes, F.D., Faria, P.R., Cardoso, S.V., Loyola, A.M., and de Sousa, S.C., Histopathology, 2015, vol. 67, no. 3, pp. 358–367.  https://doi.org/10.1111/his.12660 CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • M. S. Nazarenko
    • 1
    • 2
    Email author
  • A. V. Markov
    • 1
  • A. A. Sleptcov
    • 1
  • I. A. Koroleva
    • 1
  • D. V. Sharysh
    • 2
  • A. A. Zarubin
    • 1
  • N. R. Valiahmetov
    • 2
  • I. A. Goncharova
    • 1
  • E. F. Muslimova
    • 3
  • M. S. Kuznetsov
    • 3
  • B. N. Kozlov
    • 3
  • S. A. Afanasiev
    • 3
  • V. P. Puzyrev
    • 1
    • 2
  1. 1.Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of ScienceTomskRussia
  2. 2.Siberian State Medical UniversityTomskRussia
  3. 3.Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of ScienceTomskRussia

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