Abstract
Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Lowenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on the Lowenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained using the Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.
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Original Russian Text © J.A. Bespyatykh, O.A. Manicheva, A.V. Smolyakov, M.Z. Dogonadze, V.Yu. Zhuravlev, E.A. Shitikov, E.N. Ilina, 2018, published in Biomeditsinskaya Khimiya.
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Bespyatykh, J.A., Manicheva, O.A., Smolyakov, A.V. et al. The Influence of Cultivation Conditions on the Proteomic Profile of Mycobacterium tuberculosis H37Rv. Biochem. Moscow Suppl. Ser. B 12, 32–38 (2018). https://doi.org/10.1134/S1990750818010031
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DOI: https://doi.org/10.1134/S1990750818010031