Advertisement

The SPR-based biosensor test system for analysis of small compounds interaction with human cytochrome P450 51A1 (CYP51A1)

  • O. V. GnedenkoEmail author
  • L. A. Kaluzhskiy
  • A. A. Molnar
  • A. V. Yantsevich
  • D. V. Mukha
  • A. A. Gilep
  • S. A. Usanov
  • V. A. Stonik
  • A. S. Ivanov
  • A. V. Lisitsa
  • A. I. Archakov
Article

Abstract

The SPR-based test for human cytochrome P450 51A1 (CYP51A1) ligand screening has been developed. Applicability of this system has been validated with known azole inhibitors of cytochromes P450. The studied azoles selectively interacted with human cytochrome P450 51A1, which showed the highest affinity towards ketoconazole. The efficiency of the SPR based assay has been tested using 19 steroid and triterpene compounds, which have not been investigated as potential ligands of CYP51A1.

Keywords

human cytochrome P450 51A1 (CYP51A1) SPR-based test-system assay CYP51A1 inhibitors azoles interaction screening atherosclerosis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Taylor, F., Ward, K., Moore, T.H., Burke, M., Davey Smith, G., Casas, J.P., and Ebrahim, S. Cochrane Database Syst. Rev., 2011, vol. 1, CD004816.Google Scholar
  2. 2.
    Baker, S.K., Muscle Nerve, 2005, vol. 31, pp. 572–580.CrossRefGoogle Scholar
  3. 3.
    Korošec, T., Ačimovič, J., Seliškar, M., Kocjan, D., Fon Tacer, K., Rozman, D., and Urleb, U., Bioorg. Med. Chem., 2008, vol. 16, pp. 209–221.CrossRefGoogle Scholar
  4. 4.
    Lamb, D.C., Kelly, D.E, and Kelly, S.L., FEBS Lett., 1998, vol. 425, pp. 263–265.CrossRefGoogle Scholar
  5. 5.
    Strushkevich, N., Usanov, S.A., and Park, H.-W., J. Mol. Biol., 2010, vol. 397, pp. 1067–1078.CrossRefGoogle Scholar
  6. 6.
    Mukha, D.V., Feranchuk, S.I., Gilep, A.A., and Usanov, S.A., Biochemistry (Moscow), 2011, vol. 76, pp. 175–185.CrossRefGoogle Scholar
  7. 7.
    Bellamine, A., Mangla, A.T., Nes, W.D., and Waterman, M.R., Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 8937–8942.CrossRefGoogle Scholar
  8. 8.
    Markgren, P.-O., Hamalainen, M., and Danielson, H., Analytical Biochemistry, 1998, vol. 265, pp. 340–350.CrossRefGoogle Scholar
  9. 9.
    Ivanov, A.S., Gnedenko, O.V., Molnar, A.A., Mezentsev, Yu.V., Lisitsa, A.V., and Archakov, A.I., J. Bioinformatics and Computational Biology, 2007, vol. 5, pp. 579–592.CrossRefGoogle Scholar
  10. 10.
    Ivanov, A.S., Gnedenko, O.V., Molnar, A.A., Archakov, A.I., and Podust, L.M., ACS Chemical Biology, 2010, vol. 5, pp. 767–776.CrossRefGoogle Scholar
  11. 11.
    Ershov, P.V., Gnedenko, O.V., Molnar, A.A., Lisitsa, A.V., Ivanov, A.S., and Archakov, A.I., Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2009, vol. 3, pp. 272–288.CrossRefGoogle Scholar
  12. 12.
    Pearson, J.T., Hill, J.J., Swank, J., Isoherranen, N., Kunze, K.L., and Atkins, W.M., Biochemistry, 2006, vol. 45, pp. 6341–6353.CrossRefGoogle Scholar
  13. 13.
    Van Wauwe, J., Van Nyen, G., Coene, M.C., Stoppie, P., Cools, W., Goossens, J., Borghgraef, P., and Janssen, P.A., J. Pharmacol. Exp. Ther., 1992, vol. 261, pp. 773–779.Google Scholar
  14. 14.
    Parker, J.E., Merkamm, M., Manning, N.J., Pompon, D., Kelly, S.L., and Kelly, D.E., Antimicrob. Agents Chemother., 2008, vol. 52, pp. 3597–3603.CrossRefGoogle Scholar
  15. 15.
    Warrilow, A.G.S., Martel, C.M., Parker, J.E., Melo, N., Lamb, D.C., Nes, W.D., Kelly, D.E., and Kelly, S.L., Antimicrob. Agents Chemother., 2010, vol. 54, pp. 4235–4245.CrossRefGoogle Scholar
  16. 16.
    Biacore Sensor Surface Handbook, 2003, p. 35.Google Scholar
  17. 17.
    Majka, J. and Speck, C., Adv. Biochem. Eng. Biotechnol., 2007, vol. 104, pp. 13–36.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • O. V. Gnedenko
    • 1
    Email author
  • L. A. Kaluzhskiy
    • 1
  • A. A. Molnar
    • 1
  • A. V. Yantsevich
    • 2
  • D. V. Mukha
    • 2
  • A. A. Gilep
    • 2
  • S. A. Usanov
    • 2
  • V. A. Stonik
    • 3
  • A. S. Ivanov
    • 1
  • A. V. Lisitsa
    • 1
  • A. I. Archakov
    • 1
  1. 1.Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical SciencesMoscowRussia
  2. 2.Institute of Bioorganic Chemistry of the National Academy of Sciences of BelarusMinskBelarus
  3. 3.Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of SciencesVladivostokRussia

Personalised recommendations