Advertisement

Phenothiazines are slowly oxidizable substrates of horseradish peroxidase

  • T. V. Rogozhina
  • V. V. RogozhinEmail author
Article
  • 31 Downloads

Abstract

Reactions of peroxidase oxidation of triftazine and thioproperazine have been investigated in the presence of horseradish peroxidase using steady state kinetic methods. It has been shown that phenothiazines are slowly oxidizable substrates for horseradish peroxidase. k cat and K m values have been determined in the range of pH from 4.5 to 7.5. The study of co-oxidation of phenothiazines and o-dianisidine (ODN) revealed that in the presence of aminazine and ODN in the reaction medium both substances follow sequential oxidation. ODN oxidation was not observed until full conversion of aminazine. At pH 4.5–5.5 thioproperazine bound to the enzyme-substrate complex and caused anticompetitive inhibition of peroxidase. At pH > 5.5 sequential substrate oxidation with preferential thioproperazine conversion occurred. In the range of pH from 4.5 to 7.5 triftazine did not influence ODN oxidation.

Keywords

horseradish peroxidase triftazine thioproperazine aminazine o-dianisidine phenothiazines 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mashkovskii, M.D., Lekarstvennyie sredstva (Drug Reference Book), Moscow: Meditsina, 1972.Google Scholar
  2. 2.
    Belikov, V.G., Farmatsevticheskaya khimiya (Pharmaceutical Chemistry), Moscow: Vysshaya Shkola, 1985.Google Scholar
  3. 3.
    Chernyakov, D.K., Evdokimov, P.D., and Vishker, A.S., Lekarstvennyie sredstva v veterenarii (Drugs in Veterinary) Moscow: Kolos, 1977.Google Scholar
  4. 4.
    Arzamastsev, A.P. and Yaskina, D.S, Ultrafioletovye i infrakrasnye spektry lekarstvennykh veshchestv (Ultraviolet and Infrared Spectra of Drug Substances), Moscow: Nauka, 1975.Google Scholar
  5. 5.
    Ogewa, S., Shira, Y., and Morishima, I., Biochem. Biophys. Res. Commun., 1979, vol. 90, pp. 674–678.CrossRefGoogle Scholar
  6. 6.
    Falk, J.E., Porphyrins and Metalloporphyrins, London: Elsevier, 1964.Google Scholar
  7. 7.
    George, P., Biochem. J., 1953, vol. 54, pp. 267–276.Google Scholar
  8. 8.
    Lebedeva, O.V., Ugarova, N.N., and Berezin, I.V., Biokhimiya, 1977, vol. 42, pp. 1372–1379.Google Scholar
  9. 9.
    Berezin, I.V. and Klesov, A.A., Prakticheskyi kurs khimichesoi i fermentativnoi kinetiki, (A Practical Course of Chemical and Enzymatic Kinetics), Moscow: MSU, 1976.Google Scholar
  10. 10.
    Lebedeva, O.V. and Ugarova, N.N., Biokhimiya, 1997, vol. 62, pp. 249–253.Google Scholar
  11. 11.
    Ugarova, N.N., Lebedeva, O.V., Kurilina, T.A., and Berezin, I.V., Biokhimiya, 1977, vol. 42, pp. 1577–1584.Google Scholar
  12. 12.
    Rogozhin, V.V., Peroksidaza kak component antioksidantnoi sistemy zhivykh organizmov (Peroxidase as a Component of the Antioxidant system of Living Organisms), S.-Peters.: GIORD, 2004.Google Scholar
  13. 13.
    Dunford, H.B. and Stillman, J.S., Coord. Chem. Rev., 1976, vol. 19, pp. 187–251.CrossRefGoogle Scholar
  14. 14.
    Rogozhin, V.V. and Verkhoturov, V.V., Biochemistry (Moscow), 1998, vol. 63, pp. 661–666.Google Scholar
  15. 15.
    Ugarova, N.N., Lebedeva, O.V., and Savitskii, A.P., Peroksidaznyi kataliz i ego primenenie (Peroxidase Catalysis and Its Application), Moscow: MSU, 1981.Google Scholar
  16. 16.
    Jamada, H., Makino, R., and Jamasaki, J., Arch. Biochem. Biophys., 1975, vol. 169, pp. 344–353.CrossRefGoogle Scholar
  17. 17.
    Kutuzova, G.D., Rogozhin, V.V., Ugarova, N.N., and Berezin, I.V., Dokl. Akad. Nauk. SSSR, 1983, vol. 270, pp. 994–998.Google Scholar
  18. 18.
    Lebedeva, O.V. and Ugarova, N.N., Izv. Rus. Akad. Nauk., Ser. Khim., 1996, no.1. pp. 25–32.Google Scholar
  19. 19.
    Gazaryan, I.G., Khushpulyan, D.M., and Tishkov, V.I., Usp. Biol. Khim., 2006, vol. 46, pp. 303–322.Google Scholar
  20. 20.
    Veselova, I.A., Kireiko, A.V., and Shekhovtsova, T.N., Appl. Biochem. Microbiol., 2009, vol. 45, no. 2, pp. 125–129.CrossRefGoogle Scholar
  21. 21.
    Agha, A.E., Abbeddou, S, Makris, D.P., and Kefalas, P., Biodegradation, 2009, vol. 20, pp. 143–153.CrossRefGoogle Scholar
  22. 22.
    Walt, D.R. and Gorris, H.H., J. Amer. Chem. Soc., 2009, vol. 131, pp. 6277–6282.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Yakutsk State Agricultural AcademyYakutskRussia

Personalised recommendations