Role of Transcription Factors FoxO3 and Myogenin in Regulation of E3 Ligases MuRF-1 and MAFbx Expression in Rat Soleus at an Early Stage of Disuse Atrophy
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Hypokinesia and gravitational unloading lead to skeletal muscle atrophy resulting from alterations in the balance between anabolic and catabolic processes. Ubiquitin proteasome system plays a significant role in the process of protein breakdown in course of disuse atrophy. E3 ligases are essential components of this system. We analyzed changes in expression of E3 ligases MuRF-1 and MAFbx, as well as transcription factors FoxO3 and myogenin in rat soleus after 1 and 3 days of hindlimb unloading. We found that expression of E3 ligases was increased after 24 h of unloading, while phosphorylation of FoxO3 (S253) was decreased. However, the expression level of myogenin increased only 3 days after unloading. Our result suggests that earlier expression of MuRF-1 and MAFbx could be regulated by FoxO3, and that myogenin can also contribute to this process on the 3rd day of unloading
Keywords:ubiquitin ligases disuse myogenin
The work was supported by the Russian Foundation for Basic Research (project no. 17-04-01838).
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Conflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All procedures were performed in accordance with the European Communities Council Directive (November 24, 1986; 86/609/EEC) and the Declaration on humane treatment of animals. All procedures involving animals were approved by the Biomedicine Ethics Committee of the Institute of Biomedical Problems of the Russian Academy of Sciences/Physiology section of the Russian Bioethics Committee (protocols no. 390, 29.04.2015 and 414, 23.12.2015).
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