Induction changes in photosystems I and II in plant leaves upon modulation of membrane ion transport

  • A. A. Bulychev


The steady-state regime of linear photosynthetic electron transport implies concerted operation of photosystems I and II (PSI and PSII) in plant leaves. Acidification of the thylakoid lumen is known to cause down-regulation of PSII photochemical activity but it is not yet clear how the proton accumulation in the lumen affects the PSI activity and coordinated operation of the two photosystems in intact leaves. Chlorophyll fluorescence and absorbance of oxidized chlorophyll P700 in the near-infrared region ΔA 810–870A 810) are convenient noninvasive indicators of the redox state of PSII and PSI components, respectively. Simultaneous measurements of chlorophyll fluorescence and ΔA 810 in pea leaves revealed that some kinetic stages in the induction curves occur synchronously both in dark-adapted and preilluminated leaves. After the treatment of leaves with ionophores promoting or inhibiting the light-induced thylakoid pH gradient (valinomycin, nigericin, monensin), the induction curves of ΔA 810 and chlorophyll fluorescence were consistently modified. The results suggest that characteristic stages of ΔA 810 induction curve, representing the second and the third waves of P700 photooxidation, are closely related to ΔpH generation, although the bases of ΔpH dependence differ for these two stages. The second wave of ΔA 810 depends presumably on stroma alkalinization as a precondition for photoactivation of electron flow from PSI to terminal acceptors. The third wave of ΔA 810 is apparently due to retardation of electron flow between PSII and PSI upon acidification of the lumen.


linear electron flow thylakoid membranes proton gradient photosystems I and II ionophores redox state of P700 chlorophyll fluorescence 



absorbance difference at 810 nm and 870 nm, indicative of P700 oxidoreduction state




electron-transport chain


ferredoxin-NADP reductase


non-photochemical quenching


photosystems I and II


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  1. 1.
    Satoh K. 1982. Mechanism of photoactivation of electron transport in intact Bryopsis chloroplasts. Plant Physiol. 70, 1413–1416.PubMedCrossRefGoogle Scholar
  2. 2.
    Bulychev A.A., Niyazova M.M., Turovetsky V.B. 1985. Evidence for the delayed photoactivation of electrogenic electron transport in chloroplast membranes. Biochim. Biophys. Acta. 808, 186–191.CrossRefGoogle Scholar
  3. 3.
    Harbinson J., Hedley C.L. 1993. Changes in P-700 oxidation during the early stages of the induction of photosynthesis. Plant Physiol. 103, 649–660.PubMedGoogle Scholar
  4. 4.
    Schansker G., Srivastava A., Govindjee, Strasser R.J. 2003. Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. Funct. Plant Biol. 30, 785–796.CrossRefGoogle Scholar
  5. 5.
    Strasser R.J., Tsimilli-Michael M., Srivastava A. 2004. Analysis of the chlorophyll a transient. In: Chlorophyll a Fluorescence: A Signature of Photosynthesis. Eds. Papageorgiou G.C., Govindjee. Dordrecht, Springer, pp. 321–362.Google Scholar
  6. 6.
    Bulychev A.A., Cherkashin A.A., Rubin A.B. 2010. Dependence of chlorophyll P700 redox transients during the induction period on the transmembrane distribution of protons in chloroplasts of pea leaves. Fiziol. rastenii (Rus.). 57(1), 23–31 [Transl. version in Russ. J. Plant Physiol. 57 (1), 20–27].Google Scholar
  7. 7.
    Kramer D.M., Avenson T.J., Kanazawa A., Cruz J.A., Ivanov B., Edwards G.E. 2004. The relationship between photosynthetic electron transfer and its regulation. In: Chlorophyll a Fluorescence: A Signature of Photosynthesis. Eds. Papageorgiou G.C., Govindjee. Dordrecht, Springer, pp. 251–278.Google Scholar
  8. 8.
    Johnson G.N. 2005. Cyclic electron transport in C3 plants: Fact or artefact? J. Exp.Bot. 56, 407–416.PubMedCrossRefGoogle Scholar
  9. 9.
    Joliot P., Joliot A. 2006. Cyclic electron flow in C3 plants. Biochim. Biophys. Acta. 1757, 362–368.PubMedCrossRefGoogle Scholar
  10. 10.
    Vredenberg W.J., Bulychev A.A. 2010. Photoelectrochemical control of the balance between cyclic- and linear electron transport in photosystem I. Algorithm for P700+ induction kinetics. Biochim. Biophys. Acta. 1797, 1521–1532.PubMedCrossRefGoogle Scholar
  11. 11.
    Bukhov N., Carpentier R. 2004. Alternative Photosystem I-driven electron transport routes: Mechanisms and functions. Photosynth. Res. 82, 17–33.PubMedCrossRefGoogle Scholar
  12. 12.
    Mubarakshina M.M., Ivanov B.N. 2010. The production and scavenging of reactive oxygen species in the plastoquinone pool of chloroplast thylakoid membranes. Physiol. Plant. 140, 103–110.PubMedCrossRefGoogle Scholar
  13. 13.
    Krause G.H., Weis E. 1991. Chlorophyll fluorescence and photosynthesis: The basics. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 313–349.CrossRefGoogle Scholar
  14. 14.
    Schreiber U. 2004. Pulse-amplitude (PAM) fluorometry and saturation pulse method. In: Chlorophyll a Fluorescence: A signature of Photosynthesis. Eds. Papageorgiou G.C., Govindjee. Dordrecht, Springer, p. 279–319.Google Scholar
  15. 15.
    Pschorn R., Rühle W., Wild A. 1988. Structure and function of ferredoxin-NADP+-oxidoreductase. Photosynth. Res. 17, 217–229.CrossRefGoogle Scholar
  16. 16.
    Vredenberg W., Durchan M., Prášil O. 2009. PPhotochemical and photoelectrochemical quenching of chlorophyll fluorescence in photosystem II. Biochim. Biophys. Acta. 1787, 1468–1478.PubMedCrossRefGoogle Scholar
  17. 17.
    Klughammer C., Schreiber U. 1994. An improved method, using saturating light pulses, for the determination of photosystem I quantum yield via P700+-absorbance changes at 830 nm. Planta. 192, 261–268.CrossRefGoogle Scholar
  18. 18.
    Strasser R.J., Tsimilli-Michael M., Qiang S., Goltsev V. 2010. Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. Biochim. Biophys. Acta. 1797, 1313–1326.PubMedCrossRefGoogle Scholar
  19. 19.
    Cruz S., Goss R., Christian W., Leegood R., Horton P., Jakob T. 2011. Impact of chlororespiration on nonphotochemical quenching of chlorophyll fluorescence and on the regulation of the diadinoxanthin cycle in the diatom Thalassiosira pseudonana. J. Exp. Bot. 62, 509–519.PubMedCrossRefGoogle Scholar
  20. 20.
    Boisvert S., Joly D., Carpentier D. 2006. Quantitative analysis of the experimental O-J-I-P chlorophyll fluorescence induction kinetics: Apparent activation energy and origin of each kinetic step. FEBS J. 273, 4770–4777.PubMedCrossRefGoogle Scholar
  21. 21.
    Bulychev A.A. 1984. Different kinetics of membrane potential formation in dark-adapted and preilluminated chloroplasts. Biochim. Biophys. Acta. 766, 647–652.CrossRefGoogle Scholar
  22. 22.
    Ovchinnikov Yu.A., Ivanov V.T., Shkrob A.M. 1974. Membrane-Active Complexones. Amsterdam: Elsevier.Google Scholar
  23. 23.
    Vredenberg W.J., Bulychev A.A. 1976. Changes in the electrical potential across the thylakoid membranes of illuminated intact chloroplast in the presence of membrane-modifying agents. Plant Sci. Lett. 7, 101–107.CrossRefGoogle Scholar
  24. 24.
    Antal T.K., Osipov V., Matorin D.N., Rubin A.B. 2011. Membrane potential is involved in regulation of photosynthetic reactions in the marine diatom Thalassiosira weissflogii. J. Photochem. Photobiol. B: Biology. 102, 169–173.PubMedCrossRefGoogle Scholar

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© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Department of Biophysics, Faculty of BiologyMoscow Lomonosov State UniversityMoscowRussia

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