Analysis of NAD and NAD-Dependent Protein Deacetylation in Mouse Tissues
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Nicotinamide adenine dinucleotide (NAD) plays a key role in the vital metabolic and regulatory processes in mammals. Disturbance of the NAD level regulation is associated with the development of such serious diseases as pellagra, neurodegenerative and cardiovascular disorders, diabetes, cancer and others. This paper presents an experimental approach that allows to determine the amount of NAD+ in mouse tissues using NMR spectroscopy, as well as the level of NAD+-dependent deacetylation of proteins in the cytosol and mitochondria.
Keywords:NAD NMR spectroscopy deacetylation immunoblotting
This work was financially supported by the Russian Science Foundation, project no. 16-14-10240.
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Сonflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All experiments with animals were carried out in Petrov National Medical Research Center of Oncology in accordance with the protocol approved by the local ethics committee (abstract no. 2/197 of record no. 18 of the local ethics committee) and corresponding to the standards for working with laboratory animals.
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