Cell and Tissue Biology

, Volume 11, Issue 3, pp 227–233 | Cite as

The relationship between micelle formation and biological activity of peptide 562–572 of luteinizing hormone receptor modified with decanoyl radicals

  • E. A. Shpakova
  • V. N. Sorokoumov
  • A. V. Akent’ev
  • K. V. Derkach
  • T. B. Tennikova
  • A. O. ShpakovEmail author


Lipophilic derivatives of peptides corresponding to cytoplasmic regions of G protein-coupled receptors (GPCR) can act as intracellular agonists. Our previous work showed that peptides corresponding to residues 562–572 of luteinizing hormone receptor and modified with palmitate or decanoate at the C-terminus activate adenylate cyclase in rat testes. The stimulating effect of peptide 562–572 modified with decanoates at both the N- and the C-termini (peptide IV) reached its maximum at the peptide concentration of 10–5 M and diminished with further increase in its concentration. It was supposed that this effect was due to peptide IV ability to form micelles. To verify this hypothesis, the relationship between biological activity, hydrophobicity, and ability to form micelles was investigated for peptide IV and other acylated derivatives of peptide 562–572, including those carrying C-terminal decanoate (peptide III) and palmitate (peptide VI) moieties. It was found that the stimulating effect of peptide IV taken in the concentration of 10–5 M on adenylate cyclase activity in plasma membranes of rat testes and ovaries was only slightly lower than that of peptide VI and higher than the effect of peptide III. At the concentration of 10–3 M, the effect of peptide IV was 20–27% lower and amounted to only 50–51 and 87–88% of the effects of peptides VI and III, respectively. In spite of its high hydrophobicity, peptide IV was characterized with an abnormally low retention time when eluted from a Nucleosil C8 column during reverse-phase HPLC: it was even lower than the retention time of nonmodified peptide 562–572. However, the retention time of peptide IV, but not of other peptides, increased significantly when the eluent contained a higher proportion of trifluoroacetic acid, which disrupts micellelike structures (0.5 instead of 0.1%). The surface tension of peptide IV solution in water slightly decreased with increasing peptide concentration, but rapidly dropped and reached a plateau at the concentration of 7 × 10–6 M, which indicates the beginning of micelle formation. Thus, peptide IV in the concentrations above 10–5 M forms micelles, which prevents it from interacting with the receptor. The ability of GPCR peptides to aggregate and form micelles should be taken into account in the development of their new membrane-active analogs.


peptide luteinizing hormone receptor hydrophobicity micelle formation adenylate cyclase decanoyl radical 



adenylate cyclase


adenylyl cyclase sygnaling system


critical concentration of micelle formation


luteinizing hormone receptor


trifluoroacetic acid


G protein-coupled receptor


decanoic acid residue


palmitic acid residue


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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • E. A. Shpakova
    • 1
  • V. N. Sorokoumov
    • 1
  • A. V. Akent’ev
    • 1
  • K. V. Derkach
    • 2
  • T. B. Tennikova
    • 1
  • A. O. Shpakov
    • 2
    Email author
  1. 1.Institute of ChemistrySt. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Sechenov Institute of Evolutionary Biology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

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