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Cell and Tissue Biology

, Volume 10, Issue 2, pp 145–151 | Cite as

Dual proapoptotic and pronecrotic effect of hydrogen peroxide on human umbilical vein endothelial cells

  • A. D. NadeevEmail author
  • I. V. Kudryavtsev
  • M. K. Serebriakova
  • P. V. Avdonin
  • V. P. Zinchenko
  • N. V. Goncharov
Article

Abstract

Human umbilical vein endothelial cells were exposed in culture to hydrogen peroxide (H2O2), keeping them close to physiological conditions (high cell density, high serum content, H2O2 concentration not over 500 µM). Cell viability was assessed by flow cytometry using simultaneous staining with the fluorescent dye PO-PRO-1 to detect early apoptotic cells and DRAQ7 to detect late apoptotic and necrotic cells. The data obtained suggest that the primary mechanism of the cytotoxic response to H2O2 is apoptosis. The critical concentration of H2O2 causing death in a dense monolayer is 250 µM. Lower H2O2 concentrations (up to 200 µM) cause death of individual cells. The population of endothelial cell retains viability and response to calcium activating agonists does not change compared to control cells.

Keywords

hydrogen peroxide endothelium cytotoxicity apoptosis necrosis 

Abbreviations

ROS

reactive oxygen species

SOD

superoxide dismutase

PBS

phosphate buffered saline

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • A. D. Nadeev
    • 1
    Email author
  • I. V. Kudryavtsev
    • 2
    • 3
  • M. K. Serebriakova
    • 2
  • P. V. Avdonin
    • 4
  • V. P. Zinchenko
    • 1
  • N. V. Goncharov
    • 5
    • 6
  1. 1.Institute of Cell Biophysics, Russian Academy of SciencesPushchinoRussia
  2. 2.Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”St. PetersburgRussia
  3. 3.Far Eastern Federal UniversityVladivostokRussia
  4. 4.Koltsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  5. 5.Research Institute of HygieneOccupational Pathology and Human EcologySt. PetersburgRussia
  6. 6.Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

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