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Cell and Tissue Biology

, Volume 6, Issue 5–6, pp 412–422 | Cite as

Polyadenylated RNA and mRNA export factors in extrachromosomal nuclear domains of vitellogenic oocytes in the yellow mealworm Tenebrio molitor

  • D. S. BogolyubovEmail author
  • A. M. Kiselyov
  • S. V. Shabelnikov
  • V. N. Parfenov
Article

Abstract

The nucleus of vitellogenic oocytes of the yellow mealworm Tenebrio molitor contains a karyosphere that consists of condensed chromatin embedded in an extrachromosomal fibrogranular material. Numerous nuclear bodies located freely in the nucleoplasm are also observed. Amongst these bodies, counterparts of nuclear speckles (= interchromatin granule clusters (IGCs)) can be identified by the presence of the marker protein SC35. Microinjections of fluorescently tagged 2′-O-Me(U)22 methyl oligoribonucleotide probes, complementary to poly(A) tails of RNAs, revealed poly(A)+ RNA in the vast majority of IGCs. We found that all T. molitor oocyte IGCs contain heterogeneous ribonucleoprotein (hnRNP) core protein A1 localized to IGCs in an RNA-dependent manner. The extrachromosomal material of the karyosphere and some nucleoplasmic IGCs also contain the adapter protein Aly known to provide a link between pre-mRNA splicing and mRNA export. The essential mRNA export factor/receptor NXF1 was colocalized with Aly. In nucleoplasmic IGCs, NXF1 was found to localize in an RNA-dependent manner, whereas it was RNA-independently located in the extrachromosomal material of the karyosphere. We believe our data provide evidence for the implication of nucleoplasmic IGCs in mRNA biogenesis and retention on the path to nuclear export.

Keywords

Tenebrio molitor oocyte nucleus nuclear bodies interchromatin granule clusters laser scanning confocal microscopy 

Abbreviations

hnRNP

heterogeneous nuclear RNP

IGC

interchromatin granule cluster

FGM

fibrogranular material

NXF1

nuclear export factor 1

SC35

spliceosome component 35

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • D. S. Bogolyubov
    • 1
    Email author
  • A. M. Kiselyov
    • 1
  • S. V. Shabelnikov
    • 1
  • V. N. Parfenov
    • 1
  1. 1.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia

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