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Cell and Tissue Biology

, Volume 5, Issue 3, pp 235–242 | Cite as

Sodium butyrate induces cell senescence in transformed rodent cells resistant to apoptosis

  • Z. V. ChitikovaEmail author
  • N. D. Aksenov
  • V. A. Pospelov
  • T. V. Pospelova
Article

Abstract

The capacity of HDAC inhibitor sodium butyrate to induce senescence in cells derived from rat embryonic fibroblasts and transformed by E1A + E1B19 kDa oncogene has been studied. These transformants are resistant to apoptosis in response to γ-irradiation and the deprivation of growth factors. The process of cell senescence was investigated by analyzing cell growth curves, G1/S and G2/M cell cycle arrest and senescent associated β-galactosidase expression. The irreversibility of the antiproliferative activity of sodium butyrate was analyzed by clonogenic assay.

We show that sodium butyrate suppresses proliferation and induces senescence in the E1A + E1B19 kDa transformed cells. Interestingly, NaB induced growth arrest due to the accumulation of cells in the G2/M phase; they are not tetraploid, but mainly binuclear cells. Thus, in the case of NaB induces senescence in E1A + E1B19 kDa transformed fibroblasts, the observed suppression of cell proliferation may be a result of cytokinesis failure, which leads to the formation of binuclear and multinuclear cells incapable of proliferating.

Keywords

sodium butyrate histone deacetylase inhibitor cell senescence transformants E1A + E1B19kDa 

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Z. V. Chitikova
    • 1
    Email author
  • N. D. Aksenov
    • 1
  • V. A. Pospelov
    • 1
  • T. V. Pospelova
    • 1
  1. 1.Institute of CytologyRussian Academy of SciencesSaint-PetersburgRussia

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