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Cell and Tissue Biology

, Volume 3, Issue 3, pp 274–282 | Cite as

Characteristics of spontaneously transformed human endothelial ECV304 cell line: II. Functional responses of ECV304 cells

  • N. Ya. Gil’yanoEmail author
  • E. G. Semenova
  • R. F. Fedortseva
  • L. V. Konevega
Article

Abstract

The functional peculiarities of spontaneously transformed human endothelial ECV304 cell line were studied to estimate its adequacy as an endothelial cell model for studying angiogenesis and signal transduction. The dependence of the proliferative activity of this line on the presence of growth factors was shown. The absence of serum in the nutrition medium leads to the blockage of cells in the G1 phase of cell cycle, which is not characteristic of tumor cell lines. Low doses of beta particles emitted during the decay of the [3H]-thymidine blocked, dose-dependent proliferation of these cells in the G2/M phase. The incubation of the cells in medium with another source of β particles, 3H2O, resulted in the predominant accumulation of cells in the S phase under conditions of equal specific tritium activities. The different efficiency of β particles of tritium as a part of the H2O molecule or thymidine demonstrates that different mechanisms are responsible for different checkpoints. The checkpoint of G1/S is absent, which agrees with the presence of the deletion of chromosome 9 at locus p21. The level of NO produced by the constitutive form of NO synthase in ECV304 cells was relatively low and not modified by inducible NO-synthase inhibitors. The data obtained suggest that the ECV304 line cells retained properties of the initial spontaneously transformed cell line obtained from the human umbilical vein (HUVEC) and can be used as a model system for further studies of properties of the vascular endothelium.

Key words

ECV304 proliferation blocks of G1 S/G2 G2/M β particles flow cytometry radiometry 

Abbreviations

DAF-2 DA

4,5-diaminofluorscein diacetate

D-NAME

ω-N-nitro-D-arginine methyl ester

L-NAME

ω-N-nitro-L-arginine methyl ester

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • N. Ya. Gil’yano
    • 1
    Email author
  • E. G. Semenova
    • 2
  • R. F. Fedortseva
    • 3
  • L. V. Konevega
    • 1
  1. 1.Konstantinov Institute of Nuclear PhysicsRussian Academy of SciencesGatchinaRussia
  2. 2.Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia
  3. 3.All-Russia Center of Emergency and Radiation MedicineMinistry of Emergency SituationSt. PetersburgRussia

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