Cell and Tissue Biology

, Volume 3, Issue 3, pp 222–235

Molecular-genetic and immunophenotypic analysis of antigen profile and osteogenic and adipogenic potentials of mesenchymal stromal cells from fetal liver and adult bone marrow in rats

  • M. N. Kozhevnikova
  • A. S. Mikaelyan
  • V. I. Starostin
Article

DOI: 10.1134/S1990519X09030031

Cite this article as:
Kozhevnikova, M.N., Mikaelyan, A.S. & Starostin, V.I. Cell Tiss. Biol. (2009) 3: 222. doi:10.1134/S1990519X09030031

Abstract

Comparative characteristics of mesenchymal stromal cells (MSCs) from adult bone marrow and fetal liver are of great interest due to the similar functions performed by these organs on the organization of a hemopoietic microenvironment at various developmental periods. It is known that MSCs play a pivotal role in the formation of niches for hemopoietic stem cells. The histogenetic relation of MSCs from these two hemopoietic organs cannot be ruled out. An analysis of antigen profile using immunocytochemistry and RT-PCR has confirmed that the studied cell populations fit the MSC criteria and have no contaminations of hemopoietic, lymphoid, and endothelial cells beginning at the second passage. Comparative analysis of osteogenic and adipogenic marker expression revealed MSC from fetal liver to have a weaker potential for adipogenesis and the extremely low capability for terminal osteogenic differentiation, in contrast to pronounced osteo- and adipogenic potentials of adult bone marrow MSC. The similar cell phenotype but different differentiation potentials under identical conditions of cultivation in vitro seem to be due to different developmental programs of the pre- and postnatal histogenesis of these MSC.

Key words

mesenchymal stromal cells bone marrow fetal liver pre- and postnatal development osteogenesis adipogenesis 

Abbreviations

MSC

mesenchymal stromal cells

BM

bone marrow

FL

fetal liver

HSC

hemopoietic stem cells

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • M. N. Kozhevnikova
    • 1
  • A. S. Mikaelyan
    • 1
  • V. I. Starostin
    • 1
  1. 1.Koltzov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia

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