Role of human RAD51 recombinase in the cycle checkpoint and survival of a cell
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The RAD 51 protein, a eukaryotic homologue of Escherichia coli RecA, plays a significant role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of the Rad51 gene by specific small interfering RNA (siRNA) that inhibits cell proliferation and reduces the viability of most cells. Cells with suppressed expression of Rad51 gene have altered cell cycles and accumulate in the S and G2 phases. Our findings show that the disruption of homologous recombination leads to cell death. However, some cells, e.g., MCF-7 cells, are insensitive to the suppression of Rad51 gene expression.
Key wordsmologous recombination RAD51 cell cycle DNA repair
small interfering RNA
small interfering RNA that suppress Rad51 gene expression
human skin fibroblasts
human embryonic lung fibroblasts
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