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Neurochemical Journal

, Volume 12, Issue 4, pp 299–304 | Cite as

Interaction between Hydrogen Sulfide and Muscarinic Receptors in the Regulation of Contractility of the Mouse Atrium

  • A. S. Blokhina
  • N. N. Khaertdinov
  • A. L. Zefirov
  • G. F. SitdikovaEmail author
Experimental Articles
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Abstract

Hydrogen sulfide (H2S) is an endogenously synthesized gaseous transmitter that participates in the regulation of the cardiovascular system and has a cardioprotective effect under ischemia–reperfusion conditions. Here, we studied possible mechanisms of the interaction of H2S and muscarinic acetylcholine receptors in the regulation of mice atrium contractility in the isometric conditions. We show that sodium hydrosulfide (NaHS), an exogenous donor of H2S, caused dose-dependent and reversible depression of the contractile force in the concentration range from 1 μM to 5 mM. The negative inotropic effect of NaHS did not change after the activation of muscarinic acetylcholine receptors by carbachol. However, we observed that the negative inotropic effect of carbachol increased after preliminary application of NaHS. The application of the reducing agent dithiothreitol did not change the effects of carbachol, which indicated that the effects of NaHS was not related to a direct action on the disulfide bonds of the receptor’s protein subunits. The increased effects of carbachol after NaHS application were not prevented by the inhibition of intracellular signaling pathway that mediated activation of M-cholinergic receptors, including adenylate cyclase, guanylate cyclase, and NO-synthase. However, an increase in the carbachol negative inotropic effect was not observed when ATP-dependent potassium channels were inhibited by glibenclamide. In its turn, activation of ATPdependent potassium channels by diazoxide resulted in an increase in carbachol negative inotropic action in the atrial myocardium of mice similar to the effect of NaHS. Our data indicate that the enhanced negative inotropic effect of carbachol under the action of H2S in the mouse atrium was mediated by the activation of ATP-dependent potassium channels.

Keywords

hydrogen sulfide muscarinic acetylcholine receptors contractility mouse atria ATP-dependent potassium channels adenylate cyclase guanylate cyclase nitric oxide 

Abbreviations

H2S

hydrogen sulfide

NaHS

sodium hydrosulfide

CO

carbon monoxide

NO

nitric oxide

CBS

cystathionine β-synthase

CGL

cystathionine γ-lyase

3MST

3-mercaptopyruvate sulfurtransferase

K(ATP)-channels

ATPdependent potassium channels

M-AChR

muscarinic acetylcholine receptors

M2-AChR and M3-AChR

muscarinic acetylcholine receptors of type 2 and type 3, respectively

cGMP

cyclic guanosine monophosphate

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. S. Blokhina
    • 1
  • N. N. Khaertdinov
    • 1
  • A. L. Zefirov
    • 2
  • G. F. Sitdikova
    • 1
    • 3
    Email author
  1. 1.Kazan Federal UniversityKazanRussia
  2. 2.Kazan State Medical UniversityKazanRussia
  3. 3.KazanRussia

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