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Heterogeneous choline acetyltransferase staining in cholinergic neurons

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Abstract

We studied the features of choline acetyltransferase (ChAT) distribution in cholinergic structures of the central and peripheral nervous systems; specifically in the spinal cord and dorsal root ganglion (DRG) of embryo (E20), newborn, or adult rats using a goat polyclonal antibody. We found that this anti-ChAT antibody selectively labels both central and peripheral cholinergic structures. We observed cholinergic neurons with weak immunoreactivity. We demonstrated that at all stages of ontogeny that we studied, four groups of cholinergic neurons occurred in the cervical part of the spinal cord, viz., the small neurons of the dorsal horns, neurons of Rexed’s lamina X, neurons of the area of Rexed’s laminae VI–VII, and cells of the ventral horns. Interneurons of Rexed’s laminae VI–VII exhibited maximum ChAT reactivity as compared to other groups of cholinergic neurons of the spinal cord at all developmental stages we studied. We found an increase in the number of immunopositive cells of lamina X from E20 stage to the early postnatal and mature stages. In the peripheral cholinergic structures, i.e., the DRG, of the rat, all neurons expressed ChAT at all stages of ontogeny.

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References

  1. Dale, H.H., Feldberg, W., and Vogt, M., J. Physiol., 1936, vol. 86, no. 4, pp. 353–380.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Budantsev, A.Yu., Usp. Sovrem. Biol., 2000, no. 6, pp. 587–598.

    Google Scholar 

  3. Zakharova, E.I., Dudchenko, A.M., Svinov, M.M., Fedorova, M.M., and Germanova, E.L., Neurochem. J., 2010, vol. 4, no. 4, pp. 290–303.

    Article  Google Scholar 

  4. Klinkenberg, I., Sambeth, A., and Blokland, A., Behav. Brain Res., 2011, vol. 221, no. 2, pp. 430–442.

    Article  CAS  PubMed  Google Scholar 

  5. Micheau, J. and Marighetto, A., Behav. Brain. Res., 2011, vol. 221, no. 2, pp. 424–491.

    Article  CAS  PubMed  Google Scholar 

  6. Balentova, S., Conwell, S., and Myers, A.C., Respir. Physiol. Neurobiol., 2013, vol. 189, pp. 195–202.

    Article  CAS  PubMed  Google Scholar 

  7. Furness, J.B., in: The Rat Nervous System, Ed. Paxinos, G., San Diego: Acad. Press., 2015, pp. 61–76.

  8. Schäfer, M.K., Eiden, L.E., and Weihe, E., Neuroscience, 1998, vol. 84, pp. 361–376.

    Article  PubMed  Google Scholar 

  9. Weihe, E., Schäfer, M.K., Schütz, B., Anlauf, M., Depboylu, C., Brett, C., Chen, L., and Eiden, L.E., J. Physiol. (Paris), 1998, vol. 92, nos. 5–6, pp. 385–388.

    Article  CAS  Google Scholar 

  10. Tata, A.M., De Stefano, M.E, Tomassy, G.S., Vilaró, M.T., Levey, A.I., and Biagioni, S., J. Neurosci. Res., 2000, vol. 75, no 2, pp. 194–202.

    Article  Google Scholar 

  11. Nachmansohn, D. and Machado, A.L., J. Neurophysiol., 1943, vol. 6, pp. 397–404.

    CAS  Google Scholar 

  12. Matsumoto, M., Xie, W., Inoue, M., and Ueda, H., Mol. Pain, 2007, no. 3, pp. 41–52.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Korzhevskii, D.E., Grigor’ev, I.P., Kirik, O.V., Zelenkova, N.M., and Sukhorukova, E.G., Morfologiya, 2013, vol. 143, no. 6, pp. 69–72.

    Google Scholar 

  14. Kotsyuba, A.E. and Chertok, V.M., Tsitologiya, 2013, vol. 55, no. 11, pp. 821–827.

    CAS  Google Scholar 

  15. Shiromani, P.J., Floyd, C., and Velázquez-Moctezuma, J., Brain Res., 1990, vol. 5, pp. 317–322.

    Article  Google Scholar 

  16. Yeo, T.T., Chua-Couzens, J., Butcher, L.L., Bredesen, D.E., Cooper, J.D., Valletta, J.S., Mobley, W.C., and Longo, F.M., J. Neurosci., 1997, vol. 17, pp. 7594–7605.

    CAS  PubMed  Google Scholar 

  17. Korzhevskii, D.E., Grigor’ev, I.P., Novikova, A.D., Koval’ chuk, V.A., and Kirik, O.V., Med. Akad. Zh., 2013, vol. 13, no. 4, pp. 49–53.

    Google Scholar 

  18. Korzhevskii, D.E., Sukhorukova, E.G., Gilerovich, E.G., Petrova, E.S., Kirik, O.V., and Grigor’ev, I.P., Morfologiya, 2013, vol. 143, no. 2, pp. 81–85.

    CAS  Google Scholar 

  19. Watson, C., Paxinos, G., Kayalioglu, G., and Heise, C., Atlas of the rat spinal cord, in The Spinal Cord: A Christopher and Dana Reeve Foundation Text and Atlas, Ed. Watson, C., Paxinos, G., and Kayalioglu, G., London, 2009, pp. 238–306.

    Chapter  Google Scholar 

  20. Phelps, P.E., Barber, R.P., Brennan, L.A., Maines, V.M., Salvaterra, P.M., and Vaughn, J.E., J. Comp. Neurol., 1990, vol. 291, pp. 9–26.

    Article  CAS  PubMed  Google Scholar 

  21. Ibanez, C.F., Ernfors, P., and Persson, H., J. Neurosci. Res., 1991, vol. 29, no. 2, pp. 163–171.

    Article  CAS  PubMed  Google Scholar 

  22. Thiriet, G., Kempf, J., and Ebel, A., Int. J. Dev. Neurosci., 1992, vol. 10, pp. 459–466.

    Article  CAS  PubMed  Google Scholar 

  23. Motts, S.D., Slusarczyk, A.S., Sowick, C.S., and Schofield, B.R., Neuroscience, 2008, vol. 154, no. 1, pp. 186–195.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Mesnage, B., Gaillard, S., Godin, A.G., Rodeau, J.L., Hammer, M., Von Engelhardt, J., Wiseman, P.W., De Koninck, Y., Schlichter, R., and Cordero-Erausquin, M., J. Comp. Neurol., 2011, vol. 519, no. 16, pp. 3139–3158.

    Article  CAS  PubMed  Google Scholar 

  25. Stepien, A.E., Tripodi, M., and Arber, S., Neuron, 2010, vol. 68, no. 3, pp. 456–472.

    Article  CAS  PubMed  Google Scholar 

  26. Bertrand, S.S. and Cazalets, J., Front. Neural Circuits., 2011, vol. 5: 15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Matsuo, A., Bellier, J.P., Nishimura, M., Yasuhara, O., Saito, N., and Kimura, H., J. Biol. Chem., 2011, vol. 286, no. 7, pp. 5836–5845.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Zagoraiou, L., Akay, T., Martin, J.F., Brownstone, R.M., Jessell, T.M., and Miles, G.B., Neuron, 2009, vol. 64, pp. 645–662.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Kolos, E.A. and Korzhevskii, D.E., Morfologiya, 2013, vol. 144, no. 4, pp. 76–79.

    CAS  Google Scholar 

  30. Chiocchetti, R., Poole, D.P., Kimura, H., Aimi, Y., Robbins, H.L., Castelucci, P., and Furness, J.B., Cell Tissue Res., 2003, vol. 311, pp. 11–22.

    Article  CAS  PubMed  Google Scholar 

  31. Tooyama, I. and Kimura, H., J. Chem. Neuroanat., 2000, vol. 17, pp. 217–226.

    Article  CAS  PubMed  Google Scholar 

  32. Bellier, J.P. and Kimura, H., J. Neurochem., 2007, vol. 101, no. 6, pp. 1607–1618.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Medicine, St. Petersburg, Russia

    E. A. Kolos & D. A. Korzhevskii

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  1. E. A. Kolos
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  2. D. A. Korzhevskii
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Correspondence to E. A. Kolos.

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Original Russian Text © E.A. Kolos, D.A. Korzhevskii, 2016, published in Neirokhimiya, 2016, Vol. 33, No. 1, pp. 56–62.

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Kolos, E.A., Korzhevskii, D.A. Heterogeneous choline acetyltransferase staining in cholinergic neurons. Neurochem. J. 10, 47–52 (2016). https://doi.org/10.1134/S1819712416010104

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