Doklady Biochemistry and Biophysics

, Volume 485, Issue 1, pp 107–110 | Cite as

Principles for Construction of Bioluminescent Enzyme Biotests for Analysis of Complex Media

  • V. P. Kalyabina
  • E. N. EsimbekovaEmail author
  • I. G. Torgashina
  • K. V. Kopylova
  • V. A. Kratasyuk


In this study, we formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media, which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables, which is based on using the luminous bacteria enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.



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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • V. P. Kalyabina
    • 1
    • 2
  • E. N. Esimbekova
    • 1
    • 2
    Email author
  • I. G. Torgashina
    • 1
  • K. V. Kopylova
    • 1
  • V. A. Kratasyuk
    • 1
    • 2
  1. 1.Siberian Federal UniversityKrasnoyarskRussia
  2. 2.Institute of Biophysics, Siberian Branch of the Russian Academy of SciencesKrasnoyarskRussia

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