Doklady Biochemistry and Biophysics

, Volume 483, Issue 1, pp 374–378 | Cite as

Identification and Analysis of VTC2 Homologs Encoding the Key Enzyme of L-Ascorbic Acid Biosynthesis in Tomato Species (Solanum Section of Lycopersicon)

  • D. U. Tyapkina
  • E. Z. Kochieva
  • M. A. SluginaEmail author
Biochemistry, Biophysics, and Molecular Biology


The complete nucleotide sequences of 11 wild and cultivated tomato GDP-L-galactose-phosphorylase- 1 homologs (VTC2) were identified and characterized. The overall level of nucleotide variability was 9.19%. Totally 25 out of 54 cDNA SNPs were non-synonymous and resulted in amino acid substitutions. In the group of green-fruited tomato species, more SNPs were detected than in the group of red-fruited species, but the percentage of non-synonymous substitutions in red-fruited tomatoes was significantly higher (66.7% vs. 37.5%). In the translated amino acid sequences, the GDF-L-galactose-hexose-1-phosphate-guanyltransferase domain and the highly conserved HIT-motif were identified and a conserved motif specific for VTC2 of Solanoideae species was detected.


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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • D. U. Tyapkina
    • 1
  • E. Z. Kochieva
    • 1
    • 2
  • M. A. Slugina
    • 1
    • 2
    Email author
  1. 1.Institute of Bioengineering, Research Center of BiotechnologyRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of BiotechnologyMoscow State UniversityMoscowRussia

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