Doklady Biochemistry and Biophysics

, Volume 483, Issue 1, pp 329–332 | Cite as

Conversion of Tomato Allene Oxide Synthase LeAOS3 (CYP74C3) into Epoxyalcohol Synthase by Site-Directed Mutagenesis

  • S. S. GorinaEmail author
  • E. O. Smirnova
  • L. S. Mukhtarova
  • Y. Y. Toporkova
  • A. N. Grechkin
Biochemistry, Biophysics, and Molecular Biology


The site-directed mutagenesis of tomato allene oxide synthase LeAOS3 (CYP74C3) on the “F/L toggle” site resulted in a partial conversion of LeAOS3 into epoxyalcohol synthase.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nelson, D.R., Arch. Biochem. Biophys., 1999, vol. 369, pp. 1–10.CrossRefGoogle Scholar
  2. 2.
    Toporkova, Y.Y., Gorina, S.S., Bessolitsyna, E.K., Smirnova, E.O., Fatykhova, V.S., Bruhlmann, F., Ilyina, T.M., Mukhtarova, L.S., and Grechkin, A.N., Biochim. Biophys. Acta, 2018, vol. 1863, pp. 369–378.CrossRefGoogle Scholar
  3. 3.
    Gotoh, O., J. Biol. Chem., 1992, vol. 267, pp. 83–90.Google Scholar
  4. 4.
    Toporkova, Y.Y., Gogolev, Y.V., Mukhtarova, L.S., and Grechkin, A.N., FEBS Lett., 2008, vol. 582, pp. 3423–3428.CrossRefGoogle Scholar
  5. 5.
    Lee, D.S., Nioche, P., Hamberg, M., and Raman, C.S., Nature, 2008, vol. 455, pp. 363–370.CrossRefGoogle Scholar
  6. 6.
    Toporkova, Y.Y., Ermilova, V.S., Gorina, S.S., Mukhtarova, L.S., Osipova, E.V., and Gogolev, Y.V., FEBS Lett., 2013, vol. 587, no. 16, pp. 2552–2558.CrossRefGoogle Scholar
  7. 7.
    Toporkova, Y.Y., Osipova, E.V., Mukhtarova, L.S., Gogolev, Y.V., and Grechkin, A.N., Dokl. Biochem. Biophys., 2010, vol. 435, pp. 287–290.CrossRefGoogle Scholar
  8. 8.
    Grechkin, A.N., Bruhlmann, F., Mukhtarova, L.S., Gogolev, Y.V., and Hamberg, M., Biochim. Biophys. Acta, 2006, vol. 1761, pp. 1419–1428.CrossRefGoogle Scholar
  9. 9.
    Grechkin, A.N., Mukhtarova, L.S., Latypova, L.R., Gogolev, Y.V., Toporkova, Y.Y., and Hamberg, M., Chembiochem, 2008, vol. 9, pp. 2498–2505.CrossRefGoogle Scholar
  10. 10.
    Hamberg, M., Adv. Prostaglandin Thromboxane Leukot. Res. A, 1991, vol. 21, pp. 117–124.Google Scholar
  11. 11.
    Toporkova, Y.Y., Fatykhova, V.S., Gogolev, Y.V., Khairutdinov, B.I., Mukhtarova, L.S., and Grechkin, A.N., Biochim. Biophys. Acta, 2017, vol. 1862, pp. 167–175.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • S. S. Gorina
    • 1
    Email author
  • E. O. Smirnova
    • 1
  • L. S. Mukhtarova
    • 1
  • Y. Y. Toporkova
    • 1
  • A. N. Grechkin
    • 1
  1. 1.Kazan Institute of Biochemistry and BiophysicsFRC Kazan Scientific Center of RASMoscowRussia

Personalised recommendations