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Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10–5–1.4 × 10–2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10–11 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.

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Authors and Affiliations

  1. Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russia

    A. P. Puzyr, S. E. Medvedeva, A. E. Burov & V. S. Bondar

  2. Institute of Computational Technologies, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660049, Russia

    A. E. Burov

  3. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    Yu. P. Zernov

Authors
  1. A. P. Puzyr
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  2. S. E. Medvedeva
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  3. A. E. Burov
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  4. Yu. P. Zernov
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  5. V. S. Bondar
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Correspondence to A. P. Puzyr.

Additional information

Original Russian Text © A.P. Puzyr, S.E. Medvedeva, A.E. Burov, Yu.P. Zernov, V.S. Bondar, 2018, published in Doklady Akademii Nauk, 2018, Vol. 480, No. 6, pp. 743–746.

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Puzyr, A.P., Medvedeva, S.E., Burov, A.E. et al. Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis. Dokl Biochem Biophys 480, 173–176 (2018). https://doi.org/10.1134/S1607672918030146

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  • DOI: https://doi.org/10.1134/S1607672918030146

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