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Doklady Biochemistry and Biophysics

, Volume 480, Issue 1, pp 173–176 | Cite as

Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis

  • A. P. Puzyr
  • S. E. Medvedeva
  • A. E. Burov
  • Yu. P. Zernov
  • V. S. Bondar
Biochemistry, Biophysics, and Molecular Biology

Abstract

In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10–5–1.4 × 10–2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10–11 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. P. Puzyr
    • 1
  • S. E. Medvedeva
    • 1
  • A. E. Burov
    • 1
    • 2
  • Yu. P. Zernov
    • 3
  • V. S. Bondar
    • 1
  1. 1.Institute of Biophysics, Krasnoyarsk Research Center, Siberian BranchRussian Academy of SciencesKrasnoyarskRussia
  2. 2.Institute of Computational Technologies, Siberian BranchRussian Academy of SciencesKrasnoyarskRussia
  3. 3.Voevodsky Institute of Chemical Kinetics and Combustion, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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