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Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND–enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4°C in deionized water for 1 month. The data obtained offer the prospects for developing a new class of reusable multifunctional indicating and diagnostic test systems on the basis of MNDs and higher fungal enzymes for medical and ecological analytics.

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Authors and Affiliations

  1. Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Science, Krasnoyarsk, Russia

    O. A. Mogilnaya, N. O. Ronzhin, K. S. Artemenko & V. S. Bondar

Authors
  1. O. A. Mogilnaya
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  2. N. O. Ronzhin
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  3. K. S. Artemenko
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  4. V. S. Bondar
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Correspondence to O. A. Mogilnaya.

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Original Russian Text © O.A. Mogilnaya, N.O. Ronzhin, K.S. Artemenko, V.S. Bondar, 2018, published in Doklady Akademii Nauk, 2018, Vol. 480, No. 1, pp. 112–116.

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Mogilnaya, O.A., Ronzhin, N.O., Artemenko, K.S. et al. Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi. Dokl Biochem Biophys 480, 135–138 (2018). https://doi.org/10.1134/S160767291803002X

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  • DOI: https://doi.org/10.1134/S160767291803002X

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