Doklady Biochemistry and Biophysics

, Volume 475, Issue 1, pp 267–270 | Cite as

Expression of transcription factor genes in cell lines corresponding to different stages of pancreatic cancer progression

  • I. R. Abjalimov
  • M. V. Zinovyeva
  • L. G. Nikolaev
  • M. R. Kopantzeva
  • E. P. Kopantzev
  • E. D. Sverdlov
Biochemistry, Biophysics, and Molecular Biology
  • 56 Downloads

Abstract

The expression level of six transcription factor genes and the content of their protein products in five pancreatic cancer cell lines with parallel control of expression of three marker genes reflecting epithelial or mesenchymal state of cells was investigated. Cell lines MIA PaCa-2 and Capan-2 represented the best models of quasi-mesenchymal and epithelial, respectively, types of progression of the pancreatic ductal adenocarcinoma, according to the content of E-cadherin and vimentin and the expression of KLF5 and ZEB1 transcription factors.

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References

  1. 1.
    Kalluri, R. and Weinberg, R.A., J. Clin. Invest., 2009, vol. 119, pp. 1420–1428.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Beuran, M., Negoi, I., Paun, S., et al., Pancreatology, 2015, vol. 15, pp. 217–225.CrossRefPubMedGoogle Scholar
  3. 3.
    Diaferia, G.R., Balestrieri, C., Prosperini, E., et al., EMBO J., 2016, vol. 35, pp. 595–617.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    David, C.J., Huang, Y.H., Chen, M., et al., Cell, 2016, vol. 164, pp. 1015–1030.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Petryszak, R., Keays, M., Tang, Y.A., et al., Nucleic Acids Res., 2016, vol. 44, pp. D746–D752.CrossRefPubMedGoogle Scholar
  6. 6.
    Zheng, X., Carstens, J.L., Kim, J., et al., Nature, 2015, vol. 527, pp. 525–530.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Skvortsova, Y.V., Kondratieva, S.A., Zinovyeva, M.V., Nikolaev, L.G., Azhikina, T.L., Gainetdinov, I.V., PLoS One, 2016, vol. 11, p. e0156454.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Kopantzev, E.P., Vayshlya, N.A., Kopantseva, M.R., et al., Br. J. Cancer, 2010, vol. 102, pp. 1533–1540.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Zinovyeva M.V., Kostina M.B., Chernov I.P., Kondratyeva, L.G., and Sverdlov, E.D., Russ. J. Bioorg. Chem., 2016, vol. 42, no. 6, pp. 606–611.CrossRefGoogle Scholar
  10. 10.
    Galvan, J.A., Zlobec, I., Wartenberg, M., et al., Br. J. Cancer, 2015, vol. 112, pp. 1944–1950.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Usova E.V., Kopantzeva M.R., Egorov V.I., Kopantzev, E.P., and Sverdlov, E.D., Pat. Fiziol. Eksp. Ter., 2015, vol. 59, pp. 76–87.PubMedGoogle Scholar
  12. 12.
    Bialkowska, A.B., Crisp, M., Bannister, T., et al., Mol. Cancer Ther., 2011, vol. 10, pp. 2043–2051.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • I. R. Abjalimov
    • 1
  • M. V. Zinovyeva
    • 1
  • L. G. Nikolaev
    • 1
  • M. R. Kopantzeva
    • 1
  • E. P. Kopantzev
    • 1
  • E. D. Sverdlov
    • 1
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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