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Doklady Biochemistry and Biophysics

, Volume 475, Issue 1, pp 264–266 | Cite as

New paradoxical three-finger toxin from the cobra Naja kaouthia venom: Isolation and characterization

  • A. V. Osipov
  • A. V. Meshcheryakova
  • V. G. Starkov
  • R. Kh. Ziganshin
  • T. L. Oustitch
  • L.-E. Peters
  • V. I. Tsetlin
  • Yu. N. UtkinEmail author
Biochemistry, Biophysics, and Molecular Biology

Abstract

A new three-finger toxin nakoroxin was isolated from the cobra Naja kaouthia venom, and its complete amino acid sequence was established. Nakoroxin belongs to the group of “orphan” toxins, data on the biological activity of which are practically absent. Nakoroxin shows no cytotoxicity and does not inhibit the binding of α-bungarotoxin to nicotinic acetylcholine receptors of muscle and α7 types. However, it potentiates the binding of α-bungarotoxin to the acetylcholine-binding protein from Lymnaea stagnalis. This is the first toxin with such an unusual property.

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • A. V. Osipov
    • 1
  • A. V. Meshcheryakova
    • 1
    • 2
  • V. G. Starkov
    • 1
  • R. Kh. Ziganshin
    • 1
  • T. L. Oustitch
    • 3
  • L.-E. Peters
    • 3
  • V. I. Tsetlin
    • 1
  • Yu. N. Utkin
    • 1
    • 4
    Email author
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Department of Biomolecular SciencesWeizmann Institute of ScienceRehovotIsrael
  3. 3.Envirologix Inc.PortlandUSA
  4. 4.The National University of Science and Technology MISiSMoscowRussia

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