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Doklady Biochemistry and Biophysics

, Volume 467, Issue 1, pp 157–161 | Cite as

Expression and purification of an engineered human endothelin receptor B in a monomeric form

  • A. V. Mishin
  • A. P. Luginina
  • A. P. Potapenko
  • V. I. Borshchevskiy
  • V. Katritch
  • E. Edelweiss
  • I. S. Okhrimenko
  • V. I. Gordeliy
  • V. G. Cherezov
Biochemistry, Biophysics and Molecular Biology

Abstract

In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.

Keywords

DOKLADY Biochemistry Detergent Micelle Denaturation Curve Detergent Mixture GPCR Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • A. V. Mishin
    • 1
  • A. P. Luginina
    • 1
  • A. P. Potapenko
    • 1
  • V. I. Borshchevskiy
    • 1
    • 7
  • V. Katritch
    • 2
  • E. Edelweiss
    • 3
    • 4
  • I. S. Okhrimenko
    • 1
  • V. I. Gordeliy
    • 1
    • 4
    • 5
    • 6
    • 7
  • V. G. Cherezov
    • 1
    • 2
  1. 1.Moscow Institute of Physics and Technology (State University)Dolgoprudnyi, Moscow oblastRussia
  2. 2.Bridge Institute, Department of ChemistryUniversity of Southern CaliforniaLos AngelesUSA
  3. 3.The European Molecular Biology Laboratory (EMBL)Grenoble Cedex 9France
  4. 4.CNRSIBSGrenobleFrance
  5. 5.Universite Grenoble AlpesIBSGrenobleFrance
  6. 6.CEAIBSGrenobleFrance
  7. 7.Institute of Complex Systems (ICS), ICS-6: Molecular BiophysicsResearch Centre JuelichJuelichGermany

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