Advertisement

Doklady Biochemistry and Biophysics

, Volume 466, Issue 1, pp 47–51 | Cite as

Metagenomic bacterial community profiles of chicken embryo gastrointestinal tract by using T-RFLP analysis

  • L. A. Ilina
  • E. A. Yildirim
  • I. N. Nikonov
  • V. A. Filippova
  • G. Y. Laptev
  • N. I. Novikova
  • A. A. Grozina
  • T. N. Lenkova
  • V. A. ManukyanEmail author
  • I. A. Egorov
  • V. I. Fisinin
Biochemistry, Biophysics and Molecular Biology

Abstract

Thirty microbial phylotypes of microorganisms were found in the gastrointestinal tract of chicken belonging to the Hajseks White breed, and 38 phylotypes were found in the gastrointestinal tract of chicken belonging to the Hajseks Brown breed. The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks White breed was dominated by the typical representatives of avian intestinal microflora—bacteria of the family Enterobacteriaceae (47.3%), orders Actinomycetales (13.6%) and Bifidobacteriales (20.6%), and the family Lachnospiraceae (1.1%). The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks Brown breed was dominated by the pathogenic bacteria of the order Rickettsiales (94.8%). The metagenome of gastrointestinal tract of both breeds also contained a small number of genes of unidentified bacteria.

Keywords

Gastrointestinal Tract Terminal Restriction Fragment Length Polymorphism Chicken Embryo DOKLADY Biochemistry Lactobacillus Reuteri 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Timoshko, M.A., Mikroflora pishchevaritel’nogo trakta sel’skokhozyaistvennykh zhivotnykh (The Microflora of the Digestive Tract of Farm Animals), Chisinau: Shtiintsa, 1990.Google Scholar
  2. 2.
    Van der Wielen, P.W.J.J., Keuzenkamp, D.A., Lipman, L.J.A., van Knapen, F., and Biesterveld, S., Spatial and temporal variation of the intestinal bacterial community in commercially raised broiler chickens during growth, Microbiol. Ecol., 2002, vol. 44, pp. 286–293.CrossRefGoogle Scholar
  3. 3.
    Maiorka, A., Dahlke, F., and de Azevedo Morgulis, M.S.F., Broiler adaptation to post-hatching period, Ciencia Rural, 2006, vol. 36, pp. 701–708.CrossRefGoogle Scholar
  4. 4.
    Mead, G.C., Microbes of the avian cecum: Types present and substrates utilized, J. Exp. Zool. Suppl., 1989, vol. 3, pp. 48–54.CrossRefPubMedGoogle Scholar
  5. 5.
    Amit-Romach, E., Sklan, D., and Uni, Z., Microflora ecology of the chicken intestine using 16s ribosomal DNA primers, Poult. Sci., 2004, vol. 83, pp. 1093–1098.CrossRefPubMedGoogle Scholar
  6. 6.
    Dibner, J.J., Richards, J.D., and Knight, C.D., Microbial imprinting in gut development and health, J. Appl. Poult. Res., 2008, vol. 17, pp. 174–188.CrossRefGoogle Scholar
  7. 7.
    Kizerwetter-Swid, M. and Binek, M., Bacterial microflora of the chicken embryos and newly hatched chicken, J. Animal Feed Sci., 2008, vol. 17, pp. 224–232.Google Scholar
  8. 8.
    Babaca, Z., Isolation of bacterial pathogens from deadin-shell chicken embryos from local hatcheries, J. Veterinar. Sci. Technol., 2014, vol. 5, no. 2, pp. 170–171.Google Scholar
  9. 9.
    Rossi, D.A., Fonseca, B.B., de Melo, R.T., Silva Felipe, G., Silva, P.L., Mendonca, E.P., Filgueiras, A.L., and Beletti, M.E., Transmission of campylobacter coli in chicken embryos, Brazil. J. Microbiol., 2012, vol. 43, no. 2, pp. 535–543.CrossRefGoogle Scholar
  10. 10.
    Amann, R.I., Ludwig, W., and Schleifer, K.H., Phylogenetic identification and in situ detection of individual microbial cells without cultivation, Microbiol. Rev., 1995, vol. 59, pp. 143–169.PubMedPubMedCentralGoogle Scholar
  11. 11.
    Instruktsiya po sanitarno-mikrobiologicheskomu kontrolyu tushek, myasa ptitsy, ptitseproduktov, yaits i yaitseproduktov na ptitsevodcheskikh i pererabatyvayushchikh predpriyatiyakh (Instruction for the Sanitary-Microbiological Control of Carcasses, Poultry Meat, Poultry Products, Eggs, and Egg Products at Poultry Processing Plants), Moscow, approved by Goskomprod of USSR August 30, 1990.Google Scholar
  12. 12.
    Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab. Press, 1989.Google Scholar
  13. 13.
    Nakphaichit, M., Thanomwongwattana, S., Phraephaisarn, C., Sakamoto, N., Keawsompong, S., Nakayama, J., and Nitisinprasert, S., The effect of including Lactobacillus reuteri KUB-AC5 during posthatch feeding on the growth and ileum microbiota of broiler chickens, Poult. Sci., 2011, vol. 12, no. 90, pp. 2753–2765.CrossRefGoogle Scholar
  14. 14.
    Lowder, B.V. and Fitzgerald, J.R., Human origin for avian pathogenic Ataphylococcus aureus, Virulence, 2010, vol. 4, no. 1, pp. 283–284.CrossRefGoogle Scholar
  15. 15.
    Biberstein, E.L. and Hirsh, D.C., Bordetella in Veterinary Microbiology, Oxford, Reino Unido: Blackwell Sci., 1999.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • L. A. Ilina
    • 1
  • E. A. Yildirim
    • 1
  • I. N. Nikonov
    • 1
  • V. A. Filippova
    • 1
  • G. Y. Laptev
    • 1
  • N. I. Novikova
    • 1
  • A. A. Grozina
    • 2
  • T. N. Lenkova
    • 2
  • V. A. Manukyan
    • 2
    Email author
  • I. A. Egorov
    • 2
  • V. I. Fisinin
    • 2
  1. 1.BIOTROPH+ Ltd.St. PetersburgRussia
  2. 2.All-Russia Research and Technological Institute of PoultryMoscow oblastRussia

Personalised recommendations