Polymer Science Series B

, Volume 49, Issue 3–4, pp 93–107 | Cite as

Applicability of the critical chromatography concept to proteomics problems: Dependence of retention time on the sequence of amino acids

  • A. V. Gorshkov
  • V. V. Evreinov
  • I. A. Tarasova
  • M. V. Gorshkov
Methods of Investigations

Abstract

A peptide separation model based on the technique of liquid chromatography of macromolecules at the critical condition was proposed. In terms of this model, the array of experimental data on the separation of peptides is considered. The main phenomenological parameters of the model—effective adsorption energies of amino acid residues—were determined, thus allowing the influence of character of their alternation in the chain on retention times to be predicted. The model is applicable to investigation into the feasibility of separation in different chromatographic modes of not only peptides with the same amino acid composition and different sequences of units in the chain but also peptides containing amino acid isomers and mirror sequences with different terminal groups.

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Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • A. V. Gorshkov
    • 1
  • V. V. Evreinov
    • 1
  • I. A. Tarasova
    • 2
  • M. V. Gorshkov
    • 2
  1. 1.Semenov Institute of Chemical PhysicsRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Energy Problems of Chemical PhysicsRussian Academy of SciencesMoscowRussia

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