Preliminary Structural Characteristic of Polysaccharides Extracted From the Callus Tissue of Sosnowskyi’s Hogweed (Heracleum Sosnowskyi Manden) Stem by Aqueous Ammonium Oxalate
- 3 Downloads
Pectic polysaccharides have been isolated from callus tissue of the stem of Sosnowskyi’s hogweed (Heracleum sosnowskyi Manden) by extraction with a 0.7% aqueous solution of ammonium oxalate and characterized. It was shown that they differ from pectic polysaccharides of a native plant by a lower content of D‑galacturonic acid residues and a higher content of arabinose and galactose residues. The results obtained by a combination of ion exchange chromatography, enzymatic hydrolysis, periodate oxidation, methylation and NMR spectroscopy indicated the presence of low-methyl esterified 1,4-α-D-galacturonan, rhamogalacturonan-I, arabinan and galactan or arabinogalactan. The terminal 1,2- and 1,5-linked arabinofuranose residues, terminal, 1,3-, 1,6-linked and 3,6-substituted galactopyranose residues and 1,4-linked hexopyranose (galactopyranose or glucopyranose) residues were identified in the composition of neutral carbohydrate chains. The presence in the NMR spectra of trans-glycosyl correlation peaks of the methyl groups of 2,4-di-O-substituted rhamnose residues indirectly confirm the attachment arabinan, galactan or arabinogalactan carbohydrate chains to rhamnopyranose residues of the backbone at C-4 position.
Keywords:callus tissue pectin polysaccharides galacturonan rhamogalacturonan I arabinogalactan methylation analysis mass spectrometry NMR spectroscopy
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that there have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 1.Shakhmatov, E.A., Mikhailova, E.A., and Makarova, E.N., Chem. Var.Raw Mater., 2015, no. 4, pp. 15–22.Google Scholar
- 4.Gunter, E.A., Popeiko, O.V., and Ovodov, Yu.S., Biochemistry, 2007, vol. 72, no. 9, pp. 1238–1247.Google Scholar
- 9.Gunter, E.A., Chem. Var.Raw Mater, 2001, no. 2, pp. 63–67.Google Scholar
- 19.Sinitsyn, A.P., Chernoglazov, V.M., and Gusakov, A.V., Moscow: VINITI, 1990, vol. 25, pp. 30–37.Google Scholar