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Russian Journal of Bioorganic Chemistry

, Volume 45, Issue 6, pp 431–437 | Cite as

Systems of Delivery of CRISPR/Cas9 Ribonucleoprotein Complexes for Genome Editing

  • R. N. AmirkhanovEmail author
  • G. A. Stepanov
MINI-REVIEW
  • 19 Downloads

Abstract

The discovery of RNA-guided nucleases have enabled to leap forward in genome editing of cells and organisms. These nucleases can be delivered into cells as plasmid DNA, mRNA or ribonucleoprotein complexes (RNPs). The delivery in the form of RNP has some advantages because the target gene editing begins immediately without the process of intracellular synthesis of components and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR associated protein 9) system assembly. This strategy makes it possible to directly control RNP concentration and to decrease the number of off-targets due to rapid degradation of the complex in the cell. However, the task to develop RNP delivery systems remains unsolved. This review is devoted to RNP delivery into cells and tissues using physical approaches and different carriers. Special attention is paid to novel approaches that improve the RNP delivery efficiency.

Keywords:

CRISPR/Cas9 genome editing ribonucleoprotein complexes delivery 

Notes

FUNDING

This study was partly supported by the Ministry of Science and Higher Education of the Russian Federation (project no. 0245-2019-0001) and by the Russian Science Foundation (project no. 18-75-10069).

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of SciencesNovosibirskRussia
  2. 2.BiolabmixNovosibirskRussia
  3. 3.Novosibirsk State UniversityNovosibirskRussia

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