Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 4, pp 416–423 | Cite as

A New Iq-Peptide of the Kunitz Type from the Heteractis magnifica Sea Anemone Exhibits Neuroprotective Activity in a Model of Alzheimer’s Disease

  • A. N. Kvetkina
  • E. V. LeychenkoEmail author
  • E. A. Yurchenko
  • E. A. Pislyagin
  • S. Peigneur
  • Y. Tytgat
  • M. P. Isaeva
  • D. L. Aminin
  • E. P. Kozlovskaya


The HMIQ3c1 recombinant peptide (6434 Da), the Kunitz-type protease inhibitor, which belonged to the new group of the IQ-peptides from the Heteractis magnifica sea anemone was prepared. The inhibitory constant of trypsin by this peptide proved to be 5.0 × 10–8 M. rHMIQ3c1 was shown to have no interaction with eight isoforms of Kv1 channels. This peptide exhibited neuroprotective activity in a concentration of 10 μM, and the peptide increased the viability of cells of the Neuro2a murine neuroblastoma by 39.4 ± 6.6% in the presence of β-amyloid. However, the peptide did not influence the viability of the cells during their incubation with 6-hydroxydofamine.


sea anemone Kunitz-type protease inhibitors neuroprotective activity 



the receptor that binds α-amino-3-hydrpxy-5-methyl-4-isoxazolepropionic acid


bovine pancreatic trypsin inhibitor


potential-dependent K+-channel


receptor that binds N-метил-D-aspartate


tumor necrosis factor alpha


Transient Receptor Potential cation channel subfamily V member 1




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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. N. Kvetkina
    • 1
  • E. V. Leychenko
    • 1
    • 2
    Email author
  • E. A. Yurchenko
    • 1
  • E. A. Pislyagin
    • 1
  • S. Peigneur
    • 3
  • Y. Tytgat
    • 3
  • M. P. Isaeva
    • 1
    • 2
  • D. L. Aminin
    • 1
  • E. P. Kozlovskaya
    • 1
  1. 1.Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  2. 2.School of Natural SciencesFar Eastern Federal UniversityVladivostokRussia
  3. 3.Catholic University of LeuvenLeuvenBelgium

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