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Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 3, pp 296–301 | Cite as

The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection

  • V. V. Krasitskaya
  • A. S. Davydova
  • M. A. Vorobjeva
  • L. A. Frank
Article

Abstract

A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules.

Keywords

Са2+-regulated photoprotein obelin in vitro selection RNA aptamers 2'-fluoro-RNA 

Abbreviations

BSA

bovine serum albumin

Histag-OL

obelin elongated from the N-terminus with hexahistidine peptide

SELEX

systematic evolution of ligands by exponential enrichment

WT-OL

recombinant wild type obelin

RT-PCR

reverse transcription polymerase chain reaction

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. V. Krasitskaya
    • 1
    • 2
  • A. S. Davydova
    • 1
  • M. A. Vorobjeva
    • 1
  • L. A. Frank
    • 1
  1. 1.Institute of Biophysics Siberian Branch, Russian Academy of SciencesFRC Krasnoyarsk Science CenterKrasnoyarskRussia
  2. 2.Institute of Chemical Biology and Fundamental Medicine, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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