The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection
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A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules.
KeywordsСа2+-regulated photoprotein obelin in vitro selection RNA aptamers 2'-fluoro-RNA
bovine serum albumin
obelin elongated from the N-terminus with hexahistidine peptide
systematic evolution of ligands by exponential enrichment
recombinant wild type obelin
reverse transcription polymerase chain reaction
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- 10.Markova, S.V., Vysotski, E.S., and Lee, J., in Bioluminescence and Chemiluminescence, Case, J.F., Herring, P.J., Robison, B.H., Haddock, S.H.D., Kricka, L.J., and Stanley, P.E., Eds., Singapore: World Scientific Publishing Co., 2001, pp. 115–119.Google Scholar