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Russian Journal of Bioorganic Chemistry

, Volume 41, Issue 4, pp 451–453 | Cite as

Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction

  • E. V. Ilnitskaya
  • Yu. N. Kononevich
  • A. M. Muzafarov
  • S. A. Rzhevskiy
  • I. A. Shadrin
  • E. V. Babaev
  • V. I. Martynov
  • A. A. PakhomovEmail author
Letters to the Editor

Abstract

A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments.

Keywords

qPCR fluorescent probes chromophore BODIPY click TaqMan 

Abbreviation

FAM

fluorescein

BODIPY

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

TMB

1,3,5,7-tetramethyl-BODIPY

TBTA

tris(N-benzyltriazolylmethyl)amine

FRET

Férster resonance energy transfer

qPCR

quantitative PCR

qRT-PCR

quantitative reverse transcription PCR

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References

  1. 1.
    Ryazantsev, D.Y., Tsybulsky, D.A., Prokhorenko, I.A., Kvach, M.V., Martynenko, Y.V., Philipchenko, P.M., Shmanai, V.V., Korshun, V.A., and Zavriev, S.K., Anal. Bioanal. Chem., 2012, vol. 404, pp. 59–68.PubMedCrossRefGoogle Scholar
  2. 2.
    Horváth, P., Sebej, P., Solomek, T., and Kán, P., Org. Chem., 2015, vol. 80, pp. 1299–1311.CrossRefGoogle Scholar
  3. 3.
    Yang, M., Jalloh, A.S., Wei, W., Zhao, J., Wu, P., and Chen, P.R., Nat. Commun., 2014, vol. 5, p. 4981.PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Yu, M., Ast, S., Yu, Q., Lo, A.T., Flehr, R., Todd, M.H., and Rutledge, P.J., PLoS One, 2014, vol. 9, p. e100761. doi:  10.1371/journal.pone.0100761
  5. 5.
    Boldyrev, I.A. and Molotkovsky, Yul.G., Russ. J. Bioorg. Chem., 2006, vol. 32, pp. 78–83.CrossRefGoogle Scholar
  6. 6.
    Ustinov, A.V., Stepanova, I.A., Dubnyakova, V.V., Zatsepin, T.S., Nozhevnikova, E.V., and Korshun, V.A., Russ. J. Bioorg. Chem., 2010, vol. 36, pp. 401–445.CrossRefGoogle Scholar
  7. 7.
    Suzuki, T., Higgins, P.J., and Crawford, D.R., BioTechniques, 2000, vol. 2, pp. 332–337.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • E. V. Ilnitskaya
    • 1
  • Yu. N. Kononevich
    • 2
  • A. M. Muzafarov
    • 2
    • 3
  • S. A. Rzhevskiy
    • 4
  • I. A. Shadrin
    • 4
  • E. V. Babaev
    • 4
  • V. I. Martynov
    • 1
  • A. A. Pakhomov
    • 1
    Email author
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Nesmeyanov Institute of Organoelement CompoundsRussian Academy of SciencesMoscowRussia
  3. 3.Enikolopov Institute of Synthetic Polymeric MaterialsRussian Academy of SciencesMoscowRussia
  4. 4.Lomonosov Moscow State UniversityFaculty of ChemistryMoscowRussia

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