Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction
A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition. Comparative studies with the analogous fluorescein-based probe were carried out. We demonstrated that fluorescent probes with the TMB fluorophore can be used in qPCR experiments.
KeywordsqPCR fluorescent probes chromophore BODIPY click TaqMan
Férster resonance energy transfer
quantitative reverse transcription PCR
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