Recombinant fluorescent models for studying the diphtheria toxin
- 67 Downloads
Diphtheria toxin is a major factor of the pathogenicity of the causative agent of diphtheria Corynebacterium. Due to a small size, it is of considerable interest as the basis for the development of synthetic protein molecules with a transport function, e.g., immunotoxins. In this work we describe the expression and characterized nontoxic recombinant fluorescent derivatives of the diphtheria toxin and its nontoxic CRM197 mutant. The proteins obtained can be used for studying receptor-binding and transport functions of the toxin in cells, evaluation of the expression level of the toxin proHB-EGF receptor membranes, immunization and generation of specific antibodies against the toxin, as well as for the development of diagnostic test-systems for the diphtheria toxin and antitoxic antibodies.
Keywordsfluorescent proteins molecular cloning proHB-EGF recombinant nontoxic bacterial toxin analogues
bovine serum albumin
a lethal dose
phosphate buffered saline
precursor of the heparin-binding epidermal growth factor-like growth factor
a diphtheria toxin R domain
- SbA and SbB
diphtheria toxin A and B subunits
splicing with overlap extension PCR
Unable to display preview. Download preview PDF.
- 11.Sims, N.R.A., The Evolution of Biological Disarmament, Oxford University Press, 2001.Google Scholar