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Russian Journal of Bioorganic Chemistry

, Volume 37, Issue 7, pp 893–898 | Cite as

A quantitative assay for total fructans in burdock (Arctium spp.) roots

  • D. N. OlennikovEmail author
  • L. M. Tankhaev
Low-Molecular-Weight Compounds

Abstracts

A quantitative assay of the total content of polysaccharides (fructans) in burdock roots in fructose equivalent has been elaborated. Extraction of free carbohydrates and polysaccharides has been studied using spectrophotometry and quantitative HPTLC. The optimal parameters for extracting the target group of compounds have been determined. Metrological analysis of the elaborated assay has been performed. It has been shown that the detection error does not exceed 3%.

Keywords

burdock roots Arctium spp. fructans spectrophotometry HPTLC resorcinol assay 

Abbreviations

BAC

biologically active compound

HPTLC

high performance thin layer chromatography

Frc

fructose

Glc

glucose

MW

molecular weight

Suc

sucrose

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Institute of General and Experimental Biology, Siberian BranchRussian Academy of SciencesUlan-UdeRussia

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