A quantitative assay for total fructans in burdock (Arctium spp.) roots
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A quantitative assay of the total content of polysaccharides (fructans) in burdock roots in fructose equivalent has been elaborated. Extraction of free carbohydrates and polysaccharides has been studied using spectrophotometry and quantitative HPTLC. The optimal parameters for extracting the target group of compounds have been determined. Metrological analysis of the elaborated assay has been performed. It has been shown that the detection error does not exceed 3%.
Keywordsburdock roots Arctium spp. fructans spectrophotometry HPTLC resorcinol assay
biologically active compound
high performance thin layer chromatography
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