Russian Journal of Bioorganic Chemistry

, Volume 36, Issue 2, pp 191–198 | Cite as

The oligoclonal expansion of T cells: The investigation of its stability over time

  • A. V. Chkalina
  • I. V. Zvyagin
  • I. Z. Mamedov
  • O. V. Britanova
  • D. B. Staroverov
  • Yu. B. Lebedev
Article

Abstract

A novel experimental approach to the investigation of the repertoire of peripheral T lymphocytes of patients suffering from ankylosing spondylitis (AS) is proposed. This approach is based on the wide-range sequencing of cDNA of the β-chain of the T-cellular receptor (TcR). The results of the analysis of the diversity of sequences of the TcR antigen-binding domain (CDR3) inside the total pool of one patient with AS are presented by the example of the second V family (BV2) of TcR. The expansion of six independent TcR-expressing clones of T cells with a similar amino acid sequence of the CDR3 domains was proposed based on the results of the comparative structural analysis of the clone libraries of the cDNA of TcR BV2. The long-time stable expansion of these T clones was demonstrated during the development of the disease by specific monitoring.

Key words

repertoire of T lymphocytes oligoclonal T-cellular expansion genes of the T-cellular receptor ankylosing spondylitis 

Abbreviations

AS

ankylosing spondylitis (Bekhterev’s disease)

B-TcR

β-chain of T-cellular receptor

BV, BD, BJ, and BC

the main structural domains of the β-chain of TcR

BV2

the second TcR family (classification according to the β-chain)

CDR3

antigen-binding complementarity-determining region of TcR

RT-PCR

polymerase chain reaction with reversed transcription

TcR

T-cellular receptor

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Furmanski, A.L., Ferreira, C., Bartok, I., Dimakou, S., Rice, J., Stevenson, F.K., Millrain, M.M., Simpson, E., and Dyson, J., J. Immunol., 2008, vol. 180, pp. 1029–1039.PubMedGoogle Scholar
  2. 2.
    Davis, M. and Bjorkman, P.J., Nature, 1988, vol. 334, pp. 395–402.CrossRefPubMedGoogle Scholar
  3. 3.
    Doherty, P.C., Riberdy, J.M., and Belz, G.T., Philos. Trans. R. Soc. London, 2000, vol. 335, pp. 1093–1101.Google Scholar
  4. 4.
    Rossi, D., Franceschetti, S., Capello, D., Paoli, L.D., Lunghi, M., Conconi, A., and Gaidano, G., Am. J. Hematol., 2007, vol. 82, pp. 1103–1105.CrossRefPubMedGoogle Scholar
  5. 5.
    Smith, P., Cavenagh, J., Milne, T., Howe, D., Wilkes, S., Sinnott, P., Forster, G., and Helbert, M., J. Clin. Pathol., 2000, vol. 53, pp. 177–181.CrossRefPubMedGoogle Scholar
  6. 6.
    Moss, P., Gillespie, G., Frodsham, P., Bell, J., and Reyburn, H., Blood, 1996, vol. 87, pp. 3297–3306.PubMedGoogle Scholar
  7. 7.
    Pappas, J., Jung, W., Barda, A.K., Lin, W.L., Fincke, J.E., Purev, E., Radu, M., Gaughan, J., Helm, C.W., Hernandez, E., Freedman, R.S., and Platsoucas, C.D., Cell. Immunol., 2005, vol. 234, pp. 81–101.PubMedGoogle Scholar
  8. 8.
    Okajima, O., Wada, T., Nishida, M., Yokoyama, T., Nakayama, Y., Hashida, Y., Snibata, F., Tone, Y., Ishizaki, A., Shimizu, M., Saito, T., Ohta, K., Toma, T., and Yachie, A., Clin. Exp. Immunol., 2008, vol. 155, pp. 166–172.CrossRefPubMedGoogle Scholar
  9. 9.
    Dulhpy, N., Peyrat, M., Tieng, V., Douay, C., Rabian, C., Tamouza, R., Laoussadi, S., Berenbaum, F., Chabot, A., Bonneville, M., Charron, D., and Touberg, A., J. Immunol., 1999, vol. 162, pp. 3830–3839.Google Scholar
  10. 10.
    Carpi, M., Monti, D., Salvioli, S., Lescai, F., Pierini, M., Altilia, S., Sevini, F., Valensin, S., Ostan, R., Bucci, L., and Franceschi, C., Artificial Organs, 2006, vol. 30, pp. 730–742.CrossRefGoogle Scholar
  11. 11.
    Franceschi, C., Bonafe, M., and Valensin, S., Vaccine, 2000, vol. 18, pp. 1717–1720.CrossRefPubMedGoogle Scholar
  12. 12.
    Duftner, C., Goldberger, C., Falkenbach, A., Wurzner, R., Falkensammer, B., Pfeiffer, K.P., Maerker-Hermann, E., and Schirmer, M., Arthritis Res. Ther., 2003, vol. 5, pp. 292–300.CrossRefGoogle Scholar
  13. 13.
    Trevino, M.A., Teixeiro, E., and Bragado, R., J. Rheumatol., 2004, vol. 31, pp. 1962–1972.PubMedGoogle Scholar
  14. 14.
    Reveille, J.D., Best Practice and Research Clinical Rheumatology, 2006, vol. 20, pp. 601–609.CrossRefPubMedGoogle Scholar
  15. 15.
    Smith, A.J., Best Practice and Research Clinical Rheumatology, 2006, vol. 20, pp. 571–591.CrossRefPubMedGoogle Scholar
  16. 16.
    Brown, M.A., Curr. Opin. Rheum., 2009, vol. 21, pp. 318–323.CrossRefGoogle Scholar
  17. 17.
    Brophy, S. and Calin, A., J. Rheumatol., 2001, vol. 28, pp. 2283–2288.PubMedGoogle Scholar
  18. 18.
    Woodward, L.J. and Kam, P.C.A., Anaesthesia, 2009, vol. 64, pp. 540–554.CrossRefPubMedGoogle Scholar
  19. 19.
    Tackenberg, B., Kruth, J., Bartholomaeus, J.E., Schlege, K., Oerte, W.H., Willcox, N., Hemmer, B., and Sommer, N., Eur. J. Immunol., 2007, vol. 37, pp. 849–863.CrossRefPubMedGoogle Scholar
  20. 20.
    Scotet, E., Peyrat, M., Saulquin, X., Retiere, C., Couedel, C., Davodeau, F., Dulphy, N., Toubert, A., Bignon, J.D., Lim, A., Vie, H., Hallet, M.-M., Liblau, R., Weber, M., Berthelot, J.-M., Houssaint, E., and Bonneville, M., Eur. J. Immunol., 1999, vol. 29, pp. 973–985.CrossRefPubMedGoogle Scholar
  21. 21.
    Pfaffl, W.M., Nucleic Acid Res., 2001, vol. 29, pp. 2002–2007.Google Scholar
  22. 22.
    Mamedov, I.Z., Britanova, O.V., Chkalina, A.V., Staroverov, D.B., Amosova, A.L., Mishin, A.S., Kurnikova, M.A., Zvyagin, I.V., Mutovina, Z.Y., Gordeev, A.V., Khaidukov, S.V., Sharonov, G.V., Shagin, D.A., Chudakov, D.M., and Lebedev, Y., B, Autoimmunity, 2009, vol. 42, pp. 525–536.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. V. Chkalina
    • 1
  • I. V. Zvyagin
    • 1
  • I. Z. Mamedov
    • 1
  • O. V. Britanova
    • 1
  • D. B. Staroverov
    • 1
  • Yu. B. Lebedev
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

Personalised recommendations