Identification and expression of haponin, a new protein from HL-60 cells
We found a new protein haponin (an HLDF-like protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFβ. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E. coli cells (Rosetta™ strain) was obtained. Preparations of natural and recombinant proteins exhibited antigenic cross-reactivity to polyclonal antibodies against this peptide.
Key wordseukaryotic factor of translation initiation eIF1a haponin human promyelocytic cell line HL-60 STAT1 transcription factor
eukaryotic translation initiation factor 1a
- HLDF and HLDFβ
differentiation factors of HL-60 cells
polymerase chain reaction
signal transducer and transcription activator 1
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