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Russian Journal of Bioorganic Chemistry

, Volume 33, Issue 6, pp 607–609 | Cite as

Identification and expression of haponin, a new protein from HL-60 cells

  • A. V. Vonarshenko
  • V. V. Radchenko
  • M. V. Gapon
  • I. L. Rodionov
  • I. I. Babichenko
  • D. L. Kakuev
  • I. D. Artamonov
  • A. V. Garkovenko
  • L. G. Dyachkova
  • V. M. Lipkin
  • I. A. Kostanyan
Letters to the Editor

Abstract

We found a new protein haponin (an HLDF-like protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFβ. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E. coli cells (Rosetta™ strain) was obtained. Preparations of natural and recombinant proteins exhibited antigenic cross-reactivity to polyclonal antibodies against this peptide.

Key words

eukaryotic factor of translation initiation eIF1a haponin human promyelocytic cell line HL-60 STAT1 transcription factor 

Abbreviation

eIF1a

eukaryotic translation initiation factor 1a

haponin

HLDF-like protein

HLDF and HLDFβ

differentiation factors of HL-60 cells

PCR

polymerase chain reaction

STAT1

signal transducer and transcription activator 1

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Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • A. V. Vonarshenko
    • 1
  • V. V. Radchenko
    • 1
  • M. V. Gapon
    • 1
  • I. L. Rodionov
    • 2
  • I. I. Babichenko
    • 3
  • D. L. Kakuev
    • 1
  • I. D. Artamonov
    • 1
  • A. V. Garkovenko
    • 1
  • L. G. Dyachkova
    • 1
  • V. M. Lipkin
    • 1
  • I. A. Kostanyan
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino BranchRussian Academy of SciencesPushchino, Moscow oblastRussia
  3. 3.Department of MedicinePeople’s Friendship University of RussiaMoscowRussia

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