Russian Journal of Bioorganic Chemistry

, Volume 32, Issue 2, pp 146–153 | Cite as

Peptide fragment 66–77 of monocyte chemoattractant protein 1 and its retro-enantio analogue inhibit the migration of cells in vitro and in vivo

  • M. V. SidorovaEmail author
  • A. S. Molokoedov
  • A. A. Az’muko
  • T. I. Aref’eva
  • M. G. Melekhov
  • N. B. Kukhtina
  • T. L. Krasnikova
  • Zh. D. Bespalova
  • V. N. Bushuev


The retro-enantio-analogue of peptide 66–77 of the chemokine MCP-1 and two hexapeptide fragments 66–71 and 72–77 of the C-terminal sequence of this protein were synthesized using the Fmoc strategy of solid phase peptide synthesis. The effect of the synthetic peptides upon the MCP-1-stimulated migration of THP-1 mononuclear cells was studied in vitro. The activity of the retro-enantio analogue was found to be comparable with that of the initial peptide 66–77: both peptides inhibit the migration of monocytes and granulocytes into inflammation zones of experimental animals.

Key words

MCP-1-stimulated cell migration monocyte monocyte chemoattractant protein 1 (MCP-1) solid phase peptide synthesis synthetic peptides transpeptidation 











matrix-assisted laser desorption ionization mass spectrometry


monocyte chemoattractant protein 1




solid phase peptide synthesis


trifluoroacetic acid




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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • M. V. Sidorova
    • 1
    Email author
  • A. S. Molokoedov
    • 1
  • A. A. Az’muko
    • 1
  • T. I. Aref’eva
    • 1
  • M. G. Melekhov
    • 1
  • N. B. Kukhtina
    • 1
  • T. L. Krasnikova
    • 1
  • Zh. D. Bespalova
    • 1
  • V. N. Bushuev
    • 1
  1. 1.Federal Agency for Healthcare and Social DevelopmentRussian Cardiological Research and Production AssociationMoscowRussia

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