Russian Journal of Ecology

, Volume 44, Issue 2, pp 123–130 | Cite as

Adaptive mechanisms of nonspecific resistance to heavy metal ions in alkanotrophic actinobacteria

  • I. B. Ivshina
  • M. S. Kuyukina
  • L. V. Kostina


Sensitivity to heavy metal (HM) exposure has been studied in actinobacterial cultures maintained at the Regional Specialized Collection of Alkanotrophic Microorganisms (acronym IEGM; Possible mechanisms of nonspecific resistance of actinobacteria to HMs are discussed, which may be due to their ecological preferences, alkanotrophy, and ability to synthesize biosurfactants. Hydrocarbon-oxidizing strains have been selected that are characterized by high emulsifying activity and resistance to increased (>250 mM) HM concentrations.


alkanotrophic actinobacteria petroleum hydrocarbons emulsifying activity heavy metals nonspecific resistance biosurfactants 


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  1. Bell, J.M.L., Philp, J.C., Kuyukina, M.S., et al., Methods Evaluating Vanadium Tolerance in Bacteria Isolated from Crude Oil Contaminated Land, J. Microbiol. Methods, 2004, vol. 58, pp. 87–100.PubMedCrossRefGoogle Scholar
  2. Christofi, N. and Ivshina, I.B., Microbial Surfactants and Their Use in Field Studies of Soil Remediation, J. Appl. Microbiol., 2002, vol. 93, pp. 915–929.PubMedCrossRefGoogle Scholar
  3. Fernandes, P.J., Powell, J.A., and Archer, A.C., Construction of Rhodococcus Random Mutagenesis Libraries Using Tn5 Transposition Complexes, Microbiology, 2001, vol. 147, pp. 2529–2536.PubMedGoogle Scholar
  4. Gadd, G.M., Metal Tolerance, in Microbiology of Extreme Environments, Edwards, C., Ed., Milton Keynes, England: Open Univ. Press, 1990, pp. 178–210.Google Scholar
  5. GN Pochva, ochistka naselennykh mest, otkhody proizvodstva i potrebleniya, sanitarnaya okhrana pochvy. Predel’no dopustimye kontsentratsii (PDK) khimicheskikh veshchestv v pochve (GN Soil, Decontamination of Populated Areas, Production and Consumer Wastes, Sanitary Soil Protection: Maximum Allowable Concentrations (MACs) of Chemical Substances in the Soil), Moscow: Federal’nyi Tsentr Gossanepidnadzora Minzdrava Rossii, 2006.Google Scholar
  6. Hu, N. and Zhao, B., Key Genes Involved in Heavy-Metal Resistance in Pseudomonas putida CD2, FEMS Microbiol. Lett., 2007, vol. 267, pp. 17–22.PubMedCrossRefGoogle Scholar
  7. Ivshina, I.B. and Kuyukina, M.S., Isolation of Propane-Oxidizing Rhodococci on Selective Media with Antibiotics, Microbiology (Moscow), 1997, vol. 66, no. 4, pp. 494–500.Google Scholar
  8. Ivshina, I.B., Kamenskikh, T.N., and Anokhin, B.A., Adaptation Mechanisms Providing for the Survival of Alkanotrophic Rhodococci under Adverse Environmental Conditions, Perm Univ. Herald., Ser. Biol., 2007, no. 5 (10), pp. 113–116.Google Scholar
  9. Ivshina, I.B., Peshkur, T.A., and Korobov, V.P., Efficient Uptake of Cesium Ions by Rhodococcus Cells, Microbiology (Moscow), 2002, vol. 71, no. 3, pp. 357–361.CrossRefGoogle Scholar
  10. Ivshina, I.B., Pshenichnov, R.A., and Oborin, A.A., Propanokislyayushchie rodokokki (Propane-Oxidizing Rhodococci), Sverdlovsk: Ural. Nauch. Tsentr Akad. Nauk SSSR, 1987.Google Scholar
  11. Ivshina, I.B., Kuyukina, M.S., Philp, J.C., and Christofi, N., Oil Desorption from Mineral and Organic Materials Using Biosurfactant Complexes Produced by Rhodococcus Species, J. Microbiol. Biotechnol., 1998, vol. 14, pp. 711–717.CrossRefGoogle Scholar
  12. Regional Specialized Collection of Alkanotrophic Microorganisms: Catalog of Strains, Ivshina, I.B., Ed., Moscow: Nauka, 1994.Google Scholar
  13. Koronelli, T.V. and Yuferova, S.G., Surfactant Properties of Certain Hydrocarbon-Oxidizing Bacterial Strains, Vestn. Mosk. Gos. Univ., Ser. 16: Biol., 1990, no. 1, pp. 14–18.Google Scholar
  14. Kostina, L.V., Kuyukina, M.S., and Ivshina, I.B., Biosorbtsiya, akkumulyatsiya i sposoby izvlecheniya tyazhelykh metallov (Heavy Metals: Biosorption, Accumulation, and Methods of Recovery), Saarbrücken: Lambert Academic Publishing, 2010. 254 p.Google Scholar
  15. Kuyukina, M.S., Ivshina, I.B., Rychkova, M.I., and Chumakov, O.B., Effect of Cell Lipid Composition on the Formation of Nonspecific Antibiotic Resistance in Alkanotrophic Rhodococci, Microbiology (Moscow), 2000, vol. 69, no. 1, pp. 51–57.CrossRefGoogle Scholar
  16. Kuyukina, M.S., Ivshina, I.B., Philp, J.C., et al., Recovery of Rhodococcus Biosurfactants Using Methyl-Tertiary Butyl Ether Extraction, J. Microbiol. Methods, 2001, vol. 46, pp. 149–156.PubMedCrossRefGoogle Scholar
  17. Kvasnikov, E.I., Klyushnikova, T.M., Kasatkina, T.P., et al., Chromium-Reducing Bacteria in Nature and Industrial Wastewater Discharge, Mikrobiologiya, 1988a, vol. 57, no. 4, pp. 680–685.Google Scholar
  18. Kvasnikov, E.I., Klyushnikova, T.M., Kasatkina, T.P., et al., Resistance to Compounds of Hexavalent Chromium and Capacity for Its Reduction in Bacteria of the Genus Pseudomonas, Mikrobiol. Zh., 1988b, vol. 50, no. 6, pp. 24–27.PubMedGoogle Scholar
  19. Lurie, A., Analiticheskaya khimiya promyshlennykh vod (Analytical Chemistry of Industrial Waters), Moscow: Khimiya, 1984.Google Scholar
  20. Ochoa-Loza, F.J., Artiola, J.F., and Maier, R.M., Stability Constants for the Complexation of Various Metals with a Rhamnolipid Biosurfactant, J. Environ. Qual., 2001, vol. 30, pp. 479–485.PubMedCrossRefGoogle Scholar
  21. Philp, J.C., Kuyukina, M.S., Ivshina, I.B., et al., Alkanotrophic Rhodococcus ruber as a Biosurfactant Producer, Appl. Microbiol. Biotechnol., 2002, vol. 59, pp. 318–324.PubMedCrossRefGoogle Scholar
  22. Pimenov, E.V., Darmov, I.V., Pogorel’skii, I.P., Yanov, S.N., Kulikov, O.A., and Kalinichenko, V.B., Isolation and Characterization of Arsenic-Resistant Bacterial Strains, Microbiology (Moscow), 1996, vol. 65, no. 2, pp. 187–191.Google Scholar

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© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • I. B. Ivshina
    • 1
    • 2
  • M. S. Kuyukina
    • 1
    • 2
  • L. V. Kostina
    • 1
  1. 1.Institute of Ecology and Genetics of MicroorganismsRussian Academy of SciencesPermRussia
  2. 2.Perm State National Research UniversityPermRussia

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