Advertisement

Russian Journal of Ecology

, Volume 43, Issue 5, pp 347–351 | Cite as

AFLP analysis to estimate the genetic contribution of parents to progeny from hybridization between Saxifraga sibirica L. and S. cernua L.

  • O. S. DymshakovaEmail author
  • V. L. Semerikov
  • M. Lascoux
Article
  • 91 Downloads

Abstract

It is shown that the method of amplified fragment length polymorphism (AFLP) can be used to estimate the contribution of parent plants to the genome of the progeny from artificial crosses between Saxifraga cernua and S. sibirica. According to Nei’s (1972) genetic distances between plant groups, F1 plants are intermediate between the parent species but closer to S. cernua, probably because its genome size is twice that of S. sibirica. Conversely, B1 plants proved to be closer to S. sibirica, because the hybrid progeny were crossed back to this species.

Keywords

Saxifraga cernua S. sibirica AFLP artificial crossing hybridization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arnold, M.L., Natural Hybridization As Evolution Process, Ann. Rev. Ecol. Syst., 1992, vol. 23, pp. 237–261.CrossRefGoogle Scholar
  2. Bauert, M.R., Kaelin, M., Baltisberger, M., and Edwards, P.J., No Genetic Variation Detected within Isolated Relict Populations of the Saxifraga cernua in the Alps Using RAPD Markers, Mol. Ecol., 1998, no. 7, pp. 1519–1527.Google Scholar
  3. Bronken, P., Circumpolar Chloroplast and Nuclear DNA Variation in Saxifraga cernua, Cand. Sci. Thesis, Oslo, 2001.Google Scholar
  4. Cloutier, S., Cappadocia, M., and Landry, B.S., Analysis of RELP Mapping Inaccuracy in Brassica napus L, Theor. Appl. Genet., 1997, no. 95, pp. 83–91.Google Scholar
  5. Dymshakova, O.S., Artificial Hybridization between Saxifraga cernua L. and S. sibirica L., Russ. J. Ecol., 2009, no. 6, pp. 465–467.Google Scholar
  6. Funamoto, T., Kondo, K., Hong, D., et al., A Chromosome Study of Five Saxifraga Species Collected in Qin Ling Mountains, Shaanxi Province, China, Chromosome Sci., 1998, vol. 2, pp. 145–150.Google Scholar
  7. Gobert, V., Hybridization in the Section Mentha (Lamiaceae) Inferred from AFLP Markers, Am. J. Bot., 2002, vol. 89, no. 12, pp. 2017–2023.PubMedCrossRefGoogle Scholar
  8. Gorchakovskii, P.L., Basic Stages in the Formation of Ural Flora and Vegetation in the Cenozoic According to Paleobotanical Data: The Quaternary Period (Anthropogene), in Osnovnye problemy istoricheskoi fitogeografii Urala (Main Problems in the Historical Phytogeography of the Urals), Sverdlovsk, 1969, pp. 20–37.Google Scholar
  9. Grant, V., Plant Speciation, New York: Columbia Univ. Press, 1981. Translated under the title Vidoobrazovanie u rastenii, Moscow: Mir, 1984.Google Scholar
  10. Huff, D.R., Peacall, R., and Smouse, P.E., RAPD Variation within and among Natural Populations of Outcrossing Buffalograss Buchloe dactiloides (Nutt) Engelm., Theor. Appl. Genet., 1993, vol. 86, pp. 927–934.CrossRefGoogle Scholar
  11. Kaplan, Z. and Fehrer, J., Comparison of Natural and Artificial Hybridization in Potamogeton, Presila, 2006, vol. 78, pp. 303–316.Google Scholar
  12. Kapralov, M.V., Population Structure of the Saxifraga cernua L.-S. sibirica L. Complex in the Urals and Factors Determining It, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Yekaterinburg, 2004.Google Scholar
  13. Kapralov, M.V., Gabrielsen, T.M., Sarapultsev, I.E., et al., Genetic Enrichment of the Arctic Clonal Plant Saxifraga cernua at Its Southern Periphery via the Alpine Sexual Saxifraga sibirica, Mol. Ecol., 2006, vol. 15, pp. 3401–3411.PubMedCrossRefGoogle Scholar
  14. Li, Y.H., Han, Z.H., and Xu, X., Segregation Patterns of AFLP Markers in F1 Hybrids of a Cross Between Tetraploid and Diploid Species in the Genus Malus, Plant Breed., 2004, no. 123, pp. 316–320.Google Scholar
  15. Lowe, A.J. and Abbott, R., Routes of Origin of Two Recently Evolved Hybrid Taxa: Senecio vulgaris Var. Hibernicus and York Radiate Groundsel (Asteraceae), Am. J. Bot., 2000, vol. 87, no. 8, pp. 1159–1167.PubMedCrossRefGoogle Scholar
  16. Murín, A., Hàberovà, T., and Žamsran, C., Further Karyological Studies of the Mongolian Flora, Folia Geobot. Phytotaxon, 1984, vol. 19, pp. 29–40.Google Scholar
  17. Nei, M., Genetic Distance between Populations, Am. Nat., 1972, vol. 106, pp. 283–292.CrossRefGoogle Scholar
  18. Pan, J.T., Gornall, R., and Ohba, H., Saxifraga Linnaeus, sp. pl. 1., in Flora of China, Beijing: Sci. Press, 2001, vol. 8, pp. 280–344.Google Scholar
  19. Peakall, R. and Smouse, P.E., GenAlEx 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research, Mol. Ecol. Notes, 2006, vol. 6, pp. 288–295.CrossRefGoogle Scholar
  20. Ramsey, J. and Schemske, D.W., Pathway, Mechanisms, and Rates of Polyploid Formation in Flowering Plants, Annu. Rev. Ecol. Syst., 1998, vol. 29, pp. 267–501.CrossRefGoogle Scholar
  21. Rieseberg, L.H., Homoploid Reticulate Evolution in Helianthus (Asteraceae): Evidence from Ribosomal Genes, Am. J. Bot., 1991, vol. 78, pp. 1218–1237.CrossRefGoogle Scholar
  22. Svejda, F., Reproductive Capacity of F1 Hybrids from Rosa rugosa and chinensis Cultivars, Euphytica, 1974, vol. 23, pp. 665–669.CrossRefGoogle Scholar
  23. Tsvelev, N.N., On the Significance of Hybridization Processes in the Evolution of Grasses, in Istoriya flory i rastitel’nosti Evrazii (The History of Eurasian Flora and Vegetation), Leningrad: Nauka, 1972, pp. 5–15.Google Scholar
  24. Tsvelev, N.N., Hybridization As a Factor Increasing Biological Diversity, and Genomic Criteria of Genera in Higher Plants, Biologicheskoe raznoobrazie: podkhody k izucheniyu i sokhraneniyu: Mat-ly konf. (Proc. Conf. “Biological Diversity: Approaches to Study and Conservation,”), Bot. Inst. and Zool. Inst. Ross. Akad. Nauk, St. Petersburg, 1992, pp. 193–201.Google Scholar
  25. Vos, P., Hogers, R., Bleeker, M., et al., AFLP: A New Technique for DNA Fingerprinting, Nucleic Acids Res., 1995, vol. 23, no. 21, pp. 4407–4414.PubMedCrossRefGoogle Scholar
  26. Zhmylev, P.Yu., Hybridization in Saxifrages and Its Significance for the Evolution of the Genus Saxifraga L. (Saxifragaceae), Byull. Mosk. O-va Ispyt. Prir., Otd. Biol., 2002, vol. 107, no. 6, pp. 19–28.Google Scholar
  27. Zhukova, P.G. and Petrovskii, P.P., Karyotaxonomic Analysis of Some Species of the Genus Saxifraga (Saxifragaceae) from Northern Asia, Bot. Zh., 1987, vol. 72, no. 5, pp. 632–640.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • O. S. Dymshakova
    • 1
    Email author
  • V. L. Semerikov
    • 1
  • M. Lascoux
    • 2
  1. 1.Institute of Plant and Animal Ecology, Ural BranchRussian Academy of SciencesYekaterinburgRussia
  2. 2.Evolutionary Biology CentreUppsala UniversityUppsalaSweden

Personalised recommendations