Advertisement

Eurasian Soil Science

, Volume 48, Issue 10, pp 1101–1109 | Cite as

Preparative yield and properties of humic acids obtained by sequential alkaline extractions

  • V. A. KholodovEmail author
  • N. V. Yaroslavtseva
  • A. I. Konstantinov
  • I. V. Perminova
Soil Chemistry

Abstract

The preparative yield, composition, and structure of humic acids obtained by sequential alkaline extractions from two soils (a soddy-podzolic soil under forest and a typical chernozem in treatment with permanent black fallow of a long-term experiment since 1964) have been studied. The preparative yield of humic acids from the first extraction is 0.40 and 0.94% for the soddy-podzolic soil (Retisols) and the chernozem, respectively. The preparative yield from the second extraction is lower by several times, and the yield from the third extraction is lower by an order of magnitude. The study of the obtained preparations by elemental analysis, gel-permeation chromatography, and 13C NMR spectroscopy has shown insignificant changes in the elemental, molecular-weight, and structural-group composition of humic acids among the extractions. It has been supposed that this is related to the soil features: typical climatic factors for the formation of soil subtype in the case of soddy-podzolic soil and the land use in the long-term experiment in the case of typical chernozem. It has been concluded that that a single extraction is sufficient for the separation of humic acids and the preparation of a representative sample.

Keywords

humic preparations separation of humic substances soil organic matter long-term field experiments soddy-podzolic soils chernozems Chernozems Retisols 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    E. V. Arinushkina, Manual on the Chemical Analysis of Soils (Moscow State University, Moscow, 1970) [in Russian].Google Scholar
  2. 2.
    B. G. Belen’kii and L. Z. Vilenchik, Chromatography of Polymers (Khimiya, Moscow, 1978) [in Russian].Google Scholar
  3. 3.
    Classification and Diagnostics of Soils of the Soviet Union (Kolos, Moscow, 1977) [in Russian].Google Scholar
  4. 4.
    D. V. Kovalevskii, A. B. Permin, I. V. Perminova, and V. S. Petrosyan, “Selection of conditions of registration of quantitative 13C-NMR spectra of humic acids”, Vestn. Mosk. Univ., Ser. 2: Khim. 41 (1), 39–42 (2000).Google Scholar
  5. 5.
    B. M. Kogut, S. A. Sysuev, and V. A. Kholodov, “Water stability and labile humic substances of typical chernozems under different land uses”, Eurasian Soil Sci. 45 (5), 496–502 (2012).CrossRefGoogle Scholar
  6. 6.
    M. M. Kononova, Organic Matter of Soil (Academy of Sciences of the Soviet Union, Moscow, 1963) [in Russian].Google Scholar
  7. 7.
    D. S. Orlov and L. A. Grishina, Manual on the Chemistry of Humus (Moscow State University, Moscow, 1981) [in Russian].Google Scholar
  8. 8.
    D. S. Orlov, Humic Acids of Soils and General Theory of Humification (Moscow State University, Moscow, 1990) [in Russian].Google Scholar
  9. 9.
    I. V. Perminova, Doctoral Dissertation in Chemistry (Moscow, 2000).Google Scholar
  10. 10.
    Soil Science. Types of Soils, Their Geography and Use, Ed. by V. A. Kovda and B. G. Rozanov (Vysshaya Shkola, Moscow, 1988), Part 2.Google Scholar
  11. 11.
    V. A. Kholodov, A. I. Konstantinov, E. Yu. Belyaeva, I. V. Perminova, N. A. Kulikova, and A. V. Kiryushin, “Structure of humic acids isolated by sequential alkaline extraction from a typical chernozem”, Eurasian Soil Sci. 42 (10), 1095–1100 (2009).CrossRefGoogle Scholar
  12. 12.
    V. A. Kholodov, A. I. Konstantinov, A. V. Kudryavtsev, and I. V. Perminova, “Structure of humic acids in zonal soils from 13C NMR data”, Eurasian Soil Sci. 44 (9), 976–983 (2011).CrossRefGoogle Scholar
  13. 13.
    V. A. Kholodov, A. I. Konstantinov, and I. V. Perminova, “The carbon distribution among the functional groups of humic acids isolated by sequential alkaline extraction from gray forest soil”, Eurasian Soil Sci. 42 (11), 1229–1233 (2009).CrossRefGoogle Scholar
  14. 14.
    S. N. Chukov, “Study of humus acids in anthropogenically disturbed soils using 13C-NMR spectroscopy”, Eurasian Soil Sci. 31 (9), 979–986 (1998).Google Scholar
  15. 15.
    S. N. Chukov, Doctoral Dissertation in Biology (Moscow, 1998).Google Scholar
  16. 16.
    S. N. Chukov, V. D. Talashkina, and M. A. Nadporozhnaya, “Physiological activity of plant hormones and humic acids of soils”, Pochvovedenie, No. 2, 169–174 (1995).Google Scholar
  17. 17.
    N. Hertkorn, A. B. Permin, I. V. Perminova, D. V. Kovalevskii, M. V. Yudov, and A. Kettrup, “Comparative analysis of partial structures of a peat humic and fulvic acid using one and two dimensional nuclear magnetic resonance spectroscopy”, J. Environ. Qual. 31, 375–387 (2002).CrossRefGoogle Scholar
  18. 18.
    P. Janos, “Separation methods in the chemistry of humic substances”, J. Chromatogr. A 983, 1–18 (2003).CrossRefGoogle Scholar
  19. 19.
    A. I. Konstantinov, G. N. Vladimirov, A. S. Grigoryev, A. V. Kudryavtsev, I. V. Perminova, and E. N. Nikolaev, “Molecular composition study of mumijo from different geographic areas using size-exclusion chromatography, NMR spectroscopy, and high-resolution massspectrometry”, in Functions of Natural Organic Matter in Changing Environment (Springer-Verlag, New York, 2013), Part 1, pp. 283–287. doi 10.1007/978-94-0075634-2_52CrossRefGoogle Scholar
  20. 20.
    A. V. Kudryavtsev, I. V. Perminova, and V. S. Petrosyan, “Size-exclusion chromatographic descriptors of humic substances”, Anal. Chim. Acta 407, 193–202 (2000).CrossRefGoogle Scholar
  21. 21.
    I. V. Perminova, F. H. Frimmel, D. V. Kovalevskii, G. Abbt-Braun, A. V. Kudryavtsev, and S. Hesse, “Development of a predictive model for calculation of molecular weight of humic substances”, Water Res. 32, 872–881 (1998).CrossRefGoogle Scholar
  22. 22.
    I. V. Perminova, F. H. Frimmel, A. V. Kudryavtsev, N. A. Kulikova, G. Abbt-Braun, S. Hesse, and V. S. Petrosyan, “Molecular weight characteristics of aquatic, soil, and peat humic substances as determined by size exclusion chromatography and their statistical evaluation”, Environ. Sci. Technol. 37, 2477–2485 (2003).CrossRefGoogle Scholar
  23. 23.
    C. M. Preston, “Applications of NMR to soil organic matter analysis: history and prospects”, Soil Sci. 161, 144–166 (1996).CrossRefGoogle Scholar
  24. 24.
    R. S. Swift, “Organic matter characterization (chap. 35)”, in Methods of Soil Analysis (Soil Science Society of America, Madison, WI, 1996), Part 3, pp. 1018–1020.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • V. A. Kholodov
    • 1
    • 2
    Email author
  • N. V. Yaroslavtseva
    • 1
  • A. I. Konstantinov
    • 3
  • I. V. Perminova
    • 3
  1. 1.Dokuchaev Soil Science InstituteMoscowRussia
  2. 2.Faculty of Soil ScienceMoscow State UniversityMoscowRussia
  3. 3.Chemical FacultyMoscow State UniversityMoscowRussia

Personalised recommendations