Eurasian Soil Science

, Volume 48, Issue 10, pp 1101–1109 | Cite as

Preparative yield and properties of humic acids obtained by sequential alkaline extractions

  • V. A. KholodovEmail author
  • N. V. Yaroslavtseva
  • A. I. Konstantinov
  • I. V. Perminova
Soil Chemistry


The preparative yield, composition, and structure of humic acids obtained by sequential alkaline extractions from two soils (a soddy-podzolic soil under forest and a typical chernozem in treatment with permanent black fallow of a long-term experiment since 1964) have been studied. The preparative yield of humic acids from the first extraction is 0.40 and 0.94% for the soddy-podzolic soil (Retisols) and the chernozem, respectively. The preparative yield from the second extraction is lower by several times, and the yield from the third extraction is lower by an order of magnitude. The study of the obtained preparations by elemental analysis, gel-permeation chromatography, and 13C NMR spectroscopy has shown insignificant changes in the elemental, molecular-weight, and structural-group composition of humic acids among the extractions. It has been supposed that this is related to the soil features: typical climatic factors for the formation of soil subtype in the case of soddy-podzolic soil and the land use in the long-term experiment in the case of typical chernozem. It has been concluded that that a single extraction is sufficient for the separation of humic acids and the preparation of a representative sample.


humic preparations separation of humic substances soil organic matter long-term field experiments soddy-podzolic soils chernozems Chernozems Retisols 


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • V. A. Kholodov
    • 1
    • 2
    Email author
  • N. V. Yaroslavtseva
    • 1
  • A. I. Konstantinov
    • 3
  • I. V. Perminova
    • 3
  1. 1.Dokuchaev Soil Science InstituteMoscowRussia
  2. 2.Faculty of Soil ScienceMoscow State UniversityMoscowRussia
  3. 3.Chemical FacultyMoscow State UniversityMoscowRussia

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