Assessment of the microbial biomass using the content of phospholipids in soils of the dry steppe
Microbiological and biochemical investigations of chestnut soils and solonetzes were conducted in the dry steppe of the southern Privolzhskaya and northern Ergeni uplands. The living biomass of the microbial communities in the soils was estimated based on the content of phospholipids in the soils. Significant correlations were revealed between the contents of phospholipids and the main soil properties (the contents of humus, r = 0.66, P = 0.999; clay, r = −0.41, P = 0.95; physical clay, r = −0.57, P = 0.99; and pH, r = −0.59, P = 0.99). The content of phospholipids varied from 69 to 192 nmol/g of soil in the A1 horizons; with depth it decreased down to 36–135 in the B1 horizon and to 26–79 nmol/g of soil in the B2 horizon. The microbial biomass in the solonetzes was lower by 5 to 38% than that in the chestnut soils. A trend of the decreasing of the microbial biomass in the soils from the north to the south was revealed. Based on the content of phospholipids, the number of living microbial cells was assessed; the weighed averages of their number varied from 0.7–3.2 × 1010 to 7.5–13.6 × 1010.
Unable to display preview. Download preview PDF.
- 1.M. B. Vainshtein and E. B. Kudryashova, “Nanobacteria,” Mikrobiologiya 69(2), 163–174 (2000).Google Scholar
- 2.T. V. Ganchak and A. V. Borisov, “Content of Microscopic Fungi in Buried and Recent Soils of the Steppe Zone,” in Ecology and Soils: Lectures and Reports of the XIII All-Russian School (Ross. Akad. Nauk, Pushchino, 2006), Vol. 5, pp. 73–77 [in Russian].Google Scholar
- 3.V. A. Demkin, A. V. Borisov, and S. N. Udal’tsov, “Paleosols and Climate in the Southeast of the Central Russian Upland during the Middle and Late Bronze Ages (the 25th–15th Centuries BC),” Pochvovedenie, No. 1, 7–17 (2010) [Eur. Soil Sci. 43 (1), 5–14 (2010)].Google Scholar
- 4.N. N. Kashirskaya, T. E. Khomutova, V. V. Dmitriev, et al., “The Morphology of Cells and the Biomass of Microorganisms in the Buried Paleosols and recent Steppe Soils of the Lower Volga Region,” Pochvovedenie, No. 10, 1229–1238 (2010) [Eur. Soil Sci. 43 (10), 1140–1149 (2010)].Google Scholar
- 5.L. M. Polyanskaya and D. G. Zvyagintsev, “The Content and Composition of Microbial Biomass as an Index of the Ecological Status of Soil,” Pochvovedenie, No. 6, 706–714 (2005) [Eur. Soil Sci. 38 (6), 625–633 (2005)].Google Scholar
- 9.R. Findlay, “The Use of Phospholipid Fatty Acids to Determine Microbial Community Structure,” Molecular Microbial Ecology Manual Kluwer, Dordrecht, 1996), pp. 1–17.Google Scholar
- 11.D. I. Nikitin, Modern Methods in the Study of Microbial Ecology, Ed. by T. Rosswall, Bull. Ecol. Res. Comm. (Stockholm), 17, 85–92 (1973)Google Scholar
- 16.D. C. White and D. B. Ringelberg, Utility of the Signature Lipid Biomarker Analysis in Determining the in Situ Viable Biomass, Community Structure and Nutritional, Ed. by P. S. Amy and D. Haldeman (Lewis, Boca Raton, 1997), pp. 119–136.Google Scholar