Eurasian Soil Science

, Volume 40, Issue 8, pp 893–899 | Cite as

Mutagenic activity of heavy metals in soils of wayside slopes

  • A. I. FedorovaEmail author
  • V. N. Kalaev
  • Yu. G. Prosvirina
  • S. A. Goryainova
Degradation, Remediation, and Conservation of Soils


The genotoxic properties of soils polluted with heavy metals were studied on two wayside slopes covered with trees in the city of Voronezh. The nucleolar test in cells of the apical meristem of Zebrina pendula Schnizl. roots was used. The genotoxic effect of the soils was revealed according to the increased number of 2-and 3-nucleolar cells (from 41 to 54% and from 19 to 23% in the upper part of the first and second slopes, respectively; in the control, their number was 18 and 7%). The mean number of nucleoli per cell increased from 1.7 to 1.95 in the experiment and 1.31 in the control. The increased vehicle emissions, especially when cars go up the slopes (mainly in the upper and middle parts), correlated with the elevated heavy metal (Pb, Cu, Cd, and Zn) contents in the soil. The mutagenic substances may be removed to the Voronezh Reservoir, where they may be accumulated in some living organisms.


Heavy Metal Water Extract EURASIAN Soil Science Mutagenic Activity Genotoxic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Yu. V. Alekseev, Heavy Metals in Soils and Plants (Agropromizdat, Leningrad, 1987) [in Russian].Google Scholar
  2. 2.
    E. V. Arinushkina, Handbook on the Chemical Analysis of Soils (Mosk. Gos. Univ., Moscow, 1970) [in Russian].Google Scholar
  3. 3.
    V. V. Arkhipchuk, “Use of Nucleolus Parameters in Biotesting,” Tsitol. Genet. 29(3), 6–9 (1995).Google Scholar
  4. 4.
    V. V. Arkhipchuk, V. D. Romanenko, M. V. Arkhipchuk, et al., “Cytogenetic Method of Determining the Effect of Threshold Anthropogenic Factors on Plant and Animal Genes,” Dokl. Akad. Nauk 326(5), 908–910 (1992).Google Scholar
  5. 5.
    A. B. Belyaev, N. A. Protasova, N. S. Gorbunova, and A. A. Voronin, “Ecological Status of Chernozems in the Reserved Areas of Voronezh Oblast,” in Models and Technologies of Farming Optimization (Kursk, 2003) pp. 117–119.Google Scholar
  6. 6.
    G. P. Bespalova and Yu. A. Krotov, Maximum Permissible Concentrations of Chemical Substances in the Environment (Khimiya, Leningrad, 1985) [in Russian].Google Scholar
  7. 7.
    A. K. Butorina and V. N. Kalaev, “Analysis of Sensitivity of Different Criteria in Cytogenetic Monitoring,” Ekologiya, No. 3, 206–210 (2000).Google Scholar
  8. 8.
    N. S. Gorbunova, Extended Abstract of Candidate’s Dissertation in Biology (Voronezh, 2005).Google Scholar
  9. 9.
    E. Yu. Ivanova, “Accumulation of Genotoxic Compounds by Some Aquatic System Components of the Voronezh Reservoir,” in Biotechnology for Environmental Protection: Proceedings of the 2nd International Conference, Moscow, Russia, 2004 (Sport i Kul’tura, Moscow, 2004), Part 2, pp. 64–67 [in Russian].Google Scholar
  10. 10.
    O. B. Ivanchenko, O. N. Il’inskaya, I. I. Kostyukovich, and V. A. Skvortsova, “Assessing the Mutagenic Activity of Technogenic Soils,” in Ecological-Toxicological Characterization of the City of Kazan and the Suburban Zone (Kazan. Gos. Univ., Kazan, 1991), pp. 137–141 [in Russian].Google Scholar
  11. 11.
    V. B. Il’in, Heavy Metals in the Soil—Plant System (Nauka, Novosibirsk, 1991) [in Russian].Google Scholar
  12. 12.
    G. M. Il’kun, Atmospheric Pollutants and Plants (Naukova Dumka, Kiev, 1978) [in Russian].Google Scholar
  13. 13.
    S. G. Inge-Vechtomov, “Ecological Genetics: What Is This?,” Soros Educ. J., No. 2, 59–65 (1998).Google Scholar
  14. 14.
    V. N. Kalaev, “Comparative Characterization of Radiosensibility of Two Tradescantia Species under Irradiation with Small Doses of γ Radiation for Developing a Test Object of the Radioactive Contamination of Living Sites,” in Ecological-Physiological and Physico-Biochemical Principles of Interaction between Biosystems and the Environment (Voronezh. Gos. Univ., Voronezh, 1998), pp. 43–48 [in Russian].Google Scholar
  15. 15.
    V. A. Kovda, Basic Theory of Soils (Nauka, Moscow, 1973) [in Russian].Google Scholar
  16. 16.
    N. V. Kositsyn and N. V. Alekseeva-Popova, “Effect of Heavy Metals on Plants and Mechanisms of Their Resistance to the Effect of Metals,” in Plants under Extreme Conditions of Mineral Supply (Nauka, Leningrad, 1983), pp. 5–22 [in Russian].Google Scholar
  17. 17.
    A. A. Kos’yanenko, Quality Control of the Environment (Moscow, 1992) [in Russian].Google Scholar
  18. 18.
    V. V. Kuznetsov and G. A. Dmitrieva, Physiology of Plants (Vysshaya Shkola, Moscow, 2005) [in Russian].Google Scholar
  19. 19.
    A. A. Kulagin and Yu. A. Shagieva, Woody Plants and the Biological Conservation of Industrial Contaminations (Nauka, Moscow, 2005) [in Russian].Google Scholar
  20. 20.
    A. P. Kulaichev, Methods and Means of Data Analysis under Windows: Stage 6.0 (Informatika i Komp’yutery, Moscow, 1996) [in Russian].Google Scholar
  21. 21.
    Yu. A. Kulikov, N. S. Gorbunova, and N. A. Protasova, “Background Content of Heavy Metals in Chernozems of the Voronezh Oblast,” in Genetics, Selection, and Seed Breeding of Woody Species in the Forest-Steppe (Voronezh, 2005), pp. 59–61 [in Russian].Google Scholar
  22. 22.
    I. N. Lozanovskaya, D. S. Orlov, and L. K. Sadovnikova, Ecology and Biosphere Protection under Chemical Contamination (Vysshaya Shkola, Moscow, 1998) [in Russian].Google Scholar
  23. 23.
    G. I. Makhonina, Chemical Composition of Plants on the Ural Industrial Dumps (Ural. Gos. Univ., 1987) [in Russian].Google Scholar
  24. 24.
    T. M. Minkina, G. V. Motuzova, and O. G. Nazarenko, “Interaction of Heavy Metals with the Organic Matter of an Ordinary Chernozem,” Pochvovedenie, No. 7, 804–812 (2006) [Eur. Soil Sci. 39 (7), 720–726 (2006)].Google Scholar
  25. 25.
    Provisional Permissible Concentrations (PPCs) for Heavy Metals and Arsenic in Soils with Different Physicochemical Properties (Total Content, mg/kg) (Supplement No. 1 to the MPC and PPC List No. 6229-91. Sanitary Norm GN (Moscow, 1994) [in Russian].Google Scholar
  26. 26.
    D. P. Ormrod, “Impact of Trace Element Pollution on Plants,” in Air Pollution and Plant Life, Ed. by M. Treshow (Wiley, New York, 1984), p. 291–319.Google Scholar
  27. 27.
    V. N. Kalaev, V. M. Vakhtel’, and A. G. Babenko, RF Patent 2 237 914, 2003.Google Scholar
  28. 28.
    List of Maximum Permissible Concentrations (MPCs) and Provisional Permissible Concentrations (PPCs) for Chemical Compounds in Soil No. 6229-91 (Moscow, 1991) [in Russian].Google Scholar
  29. 29.
    N. A. Protasova and A. P. Shcherbakov, Trace Elements in Chernozems and Gray Forest Soils (Voronezh. Gos. Univ., Voronezh, 2003) [in Russian].Google Scholar
  30. 30.
    Plants under Extreme Conditions of Mineral Supply: an Ecological-Physiological Study, Ed. by M. Ya. Shkol’nik and N. V. Alekseeva-Popova (Nauka, Leningrad, 1983) [in Russian].Google Scholar
  31. 31.
    R. Rieger, A. Michaelis and M. M. Green, A Glossary of Genetics and Cytogenetics (Springer, New York, 1968).Google Scholar
  32. 32.
    V. D. Romanenko, V. V. Arkhipchuk, V. D. Solomatina, et al., “Use of Morphological Parameters of Nucleoli for Assessing Metabolic Processes in Fish Organisms,” Dokl. Akad. Nauk 326(3), 562–565 (1992).Google Scholar
  33. 33.
    M. A. Sobol’, “Role of Nucleolus in Responses of Plant Cells to Environmental Physical Factors,” Tsitol. Genet. 35(3), 72–80 (2001).Google Scholar
  34. 34.
    Resistance of Wild Species to Heavy Metals, Ed. by N. V. Alekseeva (Nauka, Leningrad) [in Russian].Google Scholar
  35. 35.
    A. I. Fedorova, N. V. Kaverina, Yu. G. Prosvirina, and E. V. Shunel’ko, “Causes of Ecogeochemical Anomalies in the Urban Environment,” in Safe Habitat Is Necessary for the Health of Population: Proceedings of the Erisman Federal Research Center of Hygiene (Voronezh, 2004), pp. 304–306 [in Russian].Google Scholar
  36. 36.
    A. I. Fedorova and A. N. Nikol’skaya, Laboratory Manual on Ecology and Nature Conservation (Vlados, Moscow, 2003) [in Russian].Google Scholar
  37. 37.
    A. I. Fedorova, Yu. G. Prosvirina, and V. N. Kalaev, “Contamination of Roadside Urban Soils on Slopes by Heavy Metals and Their Mutagenic Effect,” in Ecological and Hydrometeorological Problems of Large Cities and Industrial Zones: Proceedings of the International Scientific Conference, St. Petersburg, Russia, 2005 (St. Petersburg, 2005), pp. 30–31 [in Russian].Google Scholar
  38. 38.
    A. I. Fedorova and E. V. Shunel’ko, “Contamination of Surface Soil Horizons in Urban Voronezh Soils with Heavy Metals,” Vestn. Voronezh. Univ., Ser. Geogr., Geoekol., No. 1, 74–82 (2003).Google Scholar
  39. 39.
    A. I. Fedorova, E. V. Shunel’ko, and N. V. Kaverina, Heavy Metals in Surface Horizons of Urban Voronezh Soils and Their Effect on Plants,” in Chernozems of the Central Russia: Genesis, Geography, and Evolution: Proceedings of the International Conference, Voronezh, Russia, 2004 (Voronezh. Gos. Univ., Voronezh, 2004), pp. 259–264 [in Russian].Google Scholar
  40. 40.
    A. I. Fedorova, E. V. Shunel’ko, V. N. Kalaev, and S. A. Goryainova, “Indication of the Toxicity and Mutagenic Effect of Contaminated Soils Using Test-Plants,” in Current Problems of Soil Contamination: Proceedings of the International Conference, Moscow, Russia, 2004 (Moscow, 2004) [in Russian].Google Scholar
  41. 41.
    P. V. Chelidze and O. V. Zatsepina, “Morphofunctional Classification of Nucleoli,” Usp. Sovrem. Biol. 105(2), 252–268 (1988).Google Scholar
  42. 42.
    Yu. S. Chentsov, General Cytology (Mosk. Gos. Univ., Moscow, 1978) [in Russian].Google Scholar
  43. 43.
    E. V. Shunel’ko and A. I. Fedorova, “Ecological Evaluation of Urban Soils and the Determination of Heavy Metal Toxicity by Biotesting,” Vestn. Voronezh. Univ., Ser. Geogr., Geoekol., No. 1, 93–104 (2002).Google Scholar
  44. 44.
    A. J. Anderson, D. R. Meyer, and F. K. Mayer, “Heavy Metal Toxicities: Levels of Nickel, Cobalt, and Chromium in the Soil and Plants Associated with Visual Symptoms and Variation in Growth of an Oat Crop,” Austr. J. Agric. Res. 24(4), 557–571 (1973).CrossRefGoogle Scholar
  45. 45.
    J. Antonovics, A. D. Bradshaw, and R. G. Turner, “Heavy Metal Tolerance in Plants,” Adv. Ecol. Res., No. 1, 1–114 (1971).Google Scholar
  46. 46.
    H. E. Oberlander and K. Roth, “Die Wirkung der schwermetalle Chrom, Nickel, Kupfer, Zink, Cadmium, Quecksilber, und Blei, auf die Aufnahme und Verlagerug von Kalium und Phosphat bei jungen Gerstepflanzen,” Z. Pflanzenernaehr. Dueng. Bodenk-d. 141, 263–270 (1978).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • A. I. Fedorova
    • 1
    Email author
  • V. N. Kalaev
    • 1
  • Yu. G. Prosvirina
    • 1
  • S. A. Goryainova
    • 1
  1. 1.Voronezh State UniversityVoronezhRussia

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