In situ study of the growth and degradation processes in tetragonal lysozyme crystals on a silicon substrate by high-resolution X-ray diffractometry
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The results of an in situ study of the growth of tetragonal lysozyme crystals by high-resolution X-ray diffractometry are considered. The crystals are grown by the sitting-drop method on crystalline silicon substrates of different types: both on smooth substrates and substrates with artificial surface-relief structures using graphoepitaxy. The crystals are grown in a special hermetically closed crystallization cell, which enables one to obtain images with an optical microscope and perform in situ X-ray diffraction studies in the course of crystal growth. Measurements for lysozyme crystals were carried out in different stages of the crystallization process, including crystal nucleation and growth, developed crystals, the degradation of the crystal structure, and complete destruction.
KeywordsCrystallography Report Protein Crystal Reservoir Solution Lysozyme Crystal Crystallization Cell
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- 1.M. V. Koval’chuk, Vestn. Ross. Akad. Nauk, 73(5), 405 (2003).Google Scholar
- 2.V. I. Minkin, Izv. Ross. Akad. Nauk, Ser. Khim., No. 4, 673 (2008).Google Scholar
- 3.V. F. Razumov, Izv. Ross. Akad. Nauk, Ser. Fiz. 76(2), 223 (2012).Google Scholar
- 4.Protein Structure, Stability, and Folding, Ed. by P. Murphy (Humana Press, Totowa, New York, 2001), p. 261.Google Scholar
- 9.L. A. Zadorozhnaya, M. V. Koval’chuk, Yu. N. Shilin, et al., RF Patent, No. RU 2307204.Google Scholar
- 10.The Rigaku Journal 23, 52 (2006).Google Scholar
- 11.D. K. Bowen and B. K. Tanner, High-Resolution X-Ray Diffractometry and Topography (Taylor and Francis, London, 1998).Google Scholar