Crystallography Reports

, 56:884 | Cite as

Crystal growth of phosphopantetheine adenylyltransferase, carboxypeptidase t, and thymidine phosphorylase on the international space station by the capillary counter-diffusion method

  • I. P. KuranovaEmail author
  • E. A. Smirnova
  • Yu. A. Abramchik
  • L. A. Chupova
  • R. S. Esipov
  • V. Kh. Akparov
  • V. I. Timofeev
  • M. V. Kovalchuk
Crystal Growth


Crystals of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis, thymidine phosphorylase from Escherichia coli, carboxypeptidase T from Thermoactinomyces vulgaris and its mutant forms, and crystals of complexes of these proteins with functional ligands and inhibitors were grown by the capillary counter-diffusion method in the Japanese Experimental Module Kibo on the International Space Station. The high-resolution X-ray diffraction data sets suitable for the determination of high-resolution three-dimensional structures of these proteins were collected from the grown crystals on the SPring-8 synchrotron radiation facility. The conditions of crystal growth for the proteins and the data-collection statistics are reported. The crystals grown in microgravity diffracted to a higher resolution than crystals of the same proteins grown on Earth.


Crystallography Report International Space Station Thymidine Phosphorylase Hexamine Precipitant Solution 
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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. P. Kuranova
    • 1
    Email author
  • E. A. Smirnova
    • 1
  • Yu. A. Abramchik
    • 1
    • 2
  • L. A. Chupova
    • 2
  • R. S. Esipov
    • 2
  • V. Kh. Akparov
    • 3
  • V. I. Timofeev
    • 1
  • M. V. Kovalchuk
    • 1
    • 4
  1. 1.Shubnikov Institute of CrystallographyRussian Academy of SciencesMoscowRussia
  2. 2.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Scientific Center of Russian FederationResearch Institute for Genetics and Selection of Industrial MicroorganismsMoscowRussia
  4. 4.National Research Center Kurchatov InstituteMoscowRussia

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