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Crystallography Reports

, 56:884 | Cite as

Crystal growth of phosphopantetheine adenylyltransferase, carboxypeptidase t, and thymidine phosphorylase on the international space station by the capillary counter-diffusion method

  • I. P. KuranovaEmail author
  • E. A. Smirnova
  • Yu. A. Abramchik
  • L. A. Chupova
  • R. S. Esipov
  • V. Kh. Akparov
  • V. I. Timofeev
  • M. V. Kovalchuk
Crystal Growth

Abstract

Crystals of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis, thymidine phosphorylase from Escherichia coli, carboxypeptidase T from Thermoactinomyces vulgaris and its mutant forms, and crystals of complexes of these proteins with functional ligands and inhibitors were grown by the capillary counter-diffusion method in the Japanese Experimental Module Kibo on the International Space Station. The high-resolution X-ray diffraction data sets suitable for the determination of high-resolution three-dimensional structures of these proteins were collected from the grown crystals on the SPring-8 synchrotron radiation facility. The conditions of crystal growth for the proteins and the data-collection statistics are reported. The crystals grown in microgravity diffracted to a higher resolution than crystals of the same proteins grown on Earth.

Keywords

Crystallography Report International Space Station Thymidine Phosphorylase Hexamine Precipitant Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    A. McPherson, Crystallogr. Rev. 6(2), 157 (1996).CrossRefGoogle Scholar
  2. 2.
    I. P. Kuranova, Poverkhnost’, No. 6, 6 (2004).Google Scholar
  3. 3.
    A. A. Chernov, Acta Crystallogr. A 54, 859 (1998).CrossRefGoogle Scholar
  4. 4.
    F. Otalora, M. I. Novella, J. A. Gavira, et al., Acta Crystallogr. D 57, 412 (2001).CrossRefGoogle Scholar
  5. 5.
    H. Tanaka, T. Umehara, K. Inaka, et al., Acta Cryst. F 63, 1 (2007).CrossRefGoogle Scholar
  6. 6.
    G. E. Kundrot, R. A. Juge, M. L. Pussey, et. al., Cryst. Growth Des. 1,87.Google Scholar
  7. 7.
    E. A. Smirnova, Y. A. Kislitsyn, N. I. Sosfenov, et al., Crystallogr. Rep. 54, 901 (2009).ADSCrossRefGoogle Scholar
  8. 8.
    V. I. Timofeev, R. N. Bezuglov. V. V. Chuprov-Netochin, et al., Acta Crystallogr. F 66, 5 (2010).CrossRefGoogle Scholar
  9. 9.
    V. I. Timofeev, E. A. Smirnova, L. A. Chupova, et al., Crystallogr. Rep. 55, 1050 (2010).ADSCrossRefGoogle Scholar
  10. 10.
    V. Kh. Akparov, A. M. Grishin, M. P. Yusupova, et al., Biokhimiya 72, 515 (2007).Google Scholar
  11. 11.
    V. Kh. Akparov, A. M. Grishin, V. I. Timofeev, et al., Crystallogr. Rep. 55, 802 (2010).ADSCrossRefGoogle Scholar
  12. 12.
    R. S. Esipov, A. I. Gurevich, A. I. Miroshnikov, and D. V. Chuvikovskii, Patent No. 2188234.Google Scholar
  13. 13.
    H. Tanaka, K. Inaka, Sh. Sughiyama, J. Synchrotron Radiat 11, 45 (2004).CrossRefGoogle Scholar
  14. 14.
    S. Takahashi, T. Tsurumura, K. Aritake, et al., Acta Crystallogr. F 66, 846 (2010).CrossRefGoogle Scholar
  15. 15.
    J. M. Garcia-Ruiz and A. Moreno, Acta Crystallogr. D 50, 484 (1994).CrossRefGoogle Scholar
  16. 16.
    J. M. Garcia-Ruiz, L. A. Gonzalez-Ramirez, J. A. Gavira, et al., Acta Crystallogr. D 58, 1638 (2002).CrossRefGoogle Scholar
  17. 17.
    W. J. Cook, G. W. Koszalka, W. W. Hall, et al. J. Biol. Chem. 262, 3788 (1987).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. P. Kuranova
    • 1
    Email author
  • E. A. Smirnova
    • 1
  • Yu. A. Abramchik
    • 1
    • 2
  • L. A. Chupova
    • 2
  • R. S. Esipov
    • 2
  • V. Kh. Akparov
    • 3
  • V. I. Timofeev
    • 1
  • M. V. Kovalchuk
    • 1
    • 4
  1. 1.Shubnikov Institute of CrystallographyRussian Academy of SciencesMoscowRussia
  2. 2.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Scientific Center of Russian FederationResearch Institute for Genetics and Selection of Industrial MicroorganismsMoscowRussia
  4. 4.National Research Center Kurchatov InstituteMoscowRussia

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